外周血细胞免疫功能在胃癌患者手术前后的改变及临床意义

目的 评价胃癌患者外周血细胞免疫功能的动态变化及临床意义。方法 采用单克隆抗体方法及MTT法检测56例胃癌患者手术前后T淋巴细胞（Tlymphocyte,TC)亚群及自然杀伤（natural killer,NK)细胞活性，并与38例正常对照进行了比较。结果 术前患者CD3^＋、CD4^＋、CD4^＋／CD8^＋比值、NK细胞活性显著降低（P＜0．01），CD8^＋细胞增高（P＜0．01）；术后20天，患者细胞免疫功能逐渐恢复，与术前比较有显著性意义（P＜0．05）。Bormann Ⅰ、Ⅱ型与Ⅲ、Ⅳ型比较，淋巴结未转移与淋巴结转移者比较，术后外周血NK细胞活性、CD3^＋、CD4^ 、CD4^＋／CD8^＋比值均无显著性差异（P＞0．05）。结论 胃癌患者细胞免疫功能与肿瘤的消长有密切关系，TC亚群和NK细胞活性可作为疗效和判定预后的一个重要监测指标。

CHANGES AND CLINICAL IMPLICATION OF CELL IMMUNE FUNCTION IN PERIPHERAL BLOOD IN PREOPERATIVE AND POSTOPERATIVE PATIENTS WITH GASTRIC CANCER

Objective　To evaluate the changes and clinical implication of cell immune function in gastric cancer patients before and after operation.Methods　T lymphocyte subsets and natural killer(NK) cell activity of 56 cases of gastric cancer and 38 cases of normal subjects were measured by monoclonal anti-human T lymphocyte antibody and MTT-assay before and after operation.Results　It was found that CD+3、CD+4、 ratio of CD+4/CD+8 and NK cell activity were significantly lower, but CD+8 cell was higher in preoperative gastric cancer patients than those of the control group (P<0.01). The cell immune function gradually recovered 20 days after operation. There was significant difference before and after operation (P<0.05). No significant differences CD+3、CD+4、ratio of CD+4/CD+8 and NK cell activity were found between Borrmann Ⅰ、Ⅱ and Ⅲ、Ⅳ,lympy nodal metastasis positive and negative gastric cancer patients.Conclusion　Cell immune function in patients with gastric cancer was relates to tumor progress and T lymphocyte subsets and NK cell activity could be used as important monitoring indicators.