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Sensitivity and specificity of the Leishmania OligoC‐TesT and NASBA‐oligochromatography for diagnosis of visceral leishmaniasis in Kenya |
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| Authors: | Frank L. Basiye Margaret Mbuchi Charles Magiri George Kirigi Stijn Deborggraeve Gerard J. Schoone Alfarazdeg A. Saad Sayda El‐Safi Enock Matovu Monique K. Wasunna |
| Affiliation: | 1. Centre for Clinical Research, Kenya Medical Research Institute, Nairobi, Kenya;2. Department of Parasitology, Institute of Tropical Medicine, Antwerp, Belgium;3. Rega Institute, Katholieke Universiteit Leuven, Leuven, Belgium;4. Royal Tropical Institute, KIT Biomedical Research, Amsterdam, The Netherlands;5. Faculty of Medicine, University of Khartoum, Khartoum, Sudan;6. Faculty of Veterinary Medicine, Makerere University, Kampala, Uganda |
| Abstract: | Objective To estimate the sensitivity and specificity of the OligoC‐TesT and nucleic acid sequence‐based amplification coupled to oligochromatography (NASBA‐OC) for molecular detection of Leishmania in blood from patients with confirmed visceral leishmaniasis (VL) and healthy endemic controls from Kenya. Methods Blood specimens of 84 patients with confirmed VL and 98 endemic healthy controls from Baringo district in Kenya were submitted to both assays. Results The Leishmania OligoC‐TesT showed a sensitivity of 96.4% (95% confidence interval [CI]: 90–98.8%) and a specificity of 88.8% (95% CI: 81–93.6%), while the sensitivity and specificity of the NASBA‐OC were 79.8% (95% CI: 67–87%) and 100% (95% CI: 96.3–100%), respectively. Conclusion Our findings indicate high sensitivity of the Leishmania OligoC‐TesT on blood while the NASBA‐OC is a better marker for active disease. |
| Keywords: | visceral leishmaniasis diagnosis polymerase chain reaction nucleic acid sequence‐based amplification sensitivity specificity leishmaniose viscé rale diagnostic PCR NASBA sensibilité spé cificité leishmaniasis visceral diagnó stico PCR NASBA sensibilidad especificidad |