| 反义基因片段抑制人胶质瘤u251增殖和侵袭的体外研究 |
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| 引用本文： | 曾涛,杨卫忠,张国良,陈阵,石松生. 反义基因片段抑制人胶质瘤u251增殖和侵袭的体外研究[J]. 中国临床神经科学, 2005, 13(2): 135-139 |
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| 作者姓名： | 曾涛 杨卫忠 张国良 陈阵 石松生 |
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| 作者单位： | 福建医科大学附属协和医院神经外科,350001 |
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| 基金项目： | 福建省教育厅科研基金资助项目 (K2 0 0 1 0 85) |
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| 摘 要： | 目的:研究反义PCNA及uPAR基因片段对胶质瘤细胞株u2 5 1体外增殖和侵袭能力的影响。方法:用脂质体介导寡核苷酸转染培养的u2 5 1胶质瘤细胞,RT PCR、原位杂交和免疫组化等方法分别检测相关基因和蛋白的表达,MTT比色法研究对胶质瘤细胞增殖能力的影响,Boyden小室模型研究对细胞侵袭能力的影响。结果:PCNA及uPAR反义寡核苷酸作用u2 5 1胶质瘤细胞后相关基因的mRNA及蛋白的表达明显减弱,细胞的体外增殖和侵袭能力明显被抑制。结论:反义PCNA及uPAR寡核苷酸片段能有效地抑制胶质瘤细胞的u2 5 1相关基因的表达,并能抑制胶质瘤细胞的体外增殖和侵袭能力。
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| 关 键 词： | 反义寡核苷酸 胶质瘤 增殖细胞核抗原 尿激酶型纤维蛋白酶原激活物受体 增殖 |
| 文章编号： | 1008-0678(2005)02-0135-05 |
| 修稿时间： | 2004-12-15 |
Inhibition of Proliferative and Invasive Abilities of Glial u251 Tumor Cells in vitro by Antisense Oglionucleiotides |
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| ZENG Tao,YANG Wei-zhong,ZHANG Guo-Liang,CHEN Zhen,SHI Song-sheng. Inhibition of Proliferative and Invasive Abilities of Glial u251 Tumor Cells in vitro by Antisense Oglionucleiotides[J]. Chinese Journal of Clinical Neurosciences, 2005, 13(2): 135-139 |
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| Authors: | ZENG Tao YANG Wei-zhong ZHANG Guo-Liang CHEN Zhen SHI Song-sheng |
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| Affiliation: | ZENG Tao,YANG Wei-Zhong,ZHANG Guo-Liang,CHEN Zhen,SHI Song-Sheng Neurosurgery Department,Affiliated Union Hospital,Fujian Medical University,Fuzhou 350001,China |
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| Abstract: | Aim:To study the inhibitory effect of PCNA and uPAR asODN oligonucleiotides on cellular proliferative and invasive ability of glial tumor cell line u251. Methods: ODN was transfected into tumor cells mediated by lipofectin ,then in situ hybridization,RT-PCR and immunohistochemistry were adopted to study the expression difference of mRNA and protein after transfection, cellular proliferative and invasive abilities were assessed by MTT assay and Boyden model,respectively. Results: Both mRNA and protein expression of tumor cells reduced dramatically after transfection of asODN ,and the proliferation and invasion were inhibited significantly. Conclusion: PCNA and uPAR asODN can significantly inhibit the proliferative and invasive abilities of u251 glial tumor cells. |
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| Keywords: | antisense(as) oligodeoxyribonucleotide(ODN) glioma proliferating cell nuclear antigen(PCNA) urokinase-type plasminogen activator receptor(uPAR) proliferation |
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