视网膜光化学损伤中的前列腺素

目的研究视网膜光化学损伤中前列腺素(prostaglandin,PG)含量变化以及地塞米松(dexamethasone,DXM)对这种变化的影响。方法36只SD大鼠分为对照组和DXM组，后者于光照前3天开始，每日腹膜腔注射DXM(1 mg/kg )，连续5天。两组大鼠经(1900±106．9) lx,波长为510～560nm的光连续照射24小时，于光照前及光照结束后的6小时和1，3，7，14天分别摘除眼球，用放射免疫分析法进行视网膜前列腺素E₂(prostaglandin E₂,PGE₂)与6-酮-前列腺素F_1α(6-keto-PGF_1α)含量检测。结果对照组光照后6小时，1，3天 PGE₂的含量为(37.50±2.75，48.06±4.04，81.90±4.89)pg/ mg，6-keto-PGF_1α的含量为(4.68±0.69，7.50±0.57，10.40 ±0.71)　pg/mg,明显高于DXM组相应时间点的PGE₂(20.60±4.28，37.36 ±3.34，54.85±4.57) pg/mg及6-keto-PGF_1α(2.50±0.59，4.68±0.81，6.87±1.10) pg/mg(P<0.01)。结论DXM可抑制光化学损伤后视网膜中PG含量的升高，这对视网膜的光化学损伤可能起到改善作用。(中华眼底病杂志,1999,15:94-96)

Prostaglandins in rat retina during photochemical damage

PurposeTo evaluate the prostag landins(PG) levels and to identify the effect of dexamethasone(DXM) on PG in response to photochemical insult in rat retina.MethodsThe experiments were performed on 36 SD rats which were separated into two groups,control and treated groups,and the latter received daily intraperitoneal injections of DXM (1 mg/kg)　for 5 consecutive days,starting 3 days before light exposure.The animals were continually exposed to green fluorescent light(510-560 nm)with an illuminance level of (1900±106.9)lx for 24 hrs.The retinal concentration of PGE 2 and 6-keto-PGF_1α were tested at 6hrs,1,3,7 and 14 days after light exposure.　ResultsThe PGE₂ and 6-keto-PGF_1α levels of the control groups (37.50±2.75,48.06±4.0 4,81.90±4.89) pg/mg and (4.68±0.69,7.50±0.57,10.40±0.71) pg/mg had significantly higher values than those of the treated rats(20.60±4.28,37.36± 3.34,54.85±4.57) pg/mg and (2.50±0.59,4．68±0.81,6.87±1.10)pg/mg (P<0.01) after 6 hrs,1 and 3 days light exposure respectively.ConclusionBy inhibition of PG synthesis,the DXM may play an ameliorative effect on retinal photochemical injury of rats.(Chin J Ocul Fundus Dis,1999,15:94-96)