Insulin does not activate a phosphoinositide-specific phospholipase C in adipocytes |
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Authors: | R Etindi J N Fain |
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Affiliation: | Department of Biochemistry, University of Tennessee, Memphis 38163. |
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Abstract: | Phosphoinositide-specific phospholipase C (PI-PLC) activity was determined in homogenates of adipocytes treated with maximal concentrations of insulin. PI-PLC activity measured using exogenous [3H]phosphatidylinositol [( 3H]PI) and exogenous [3H]phosphatidylinositol 4,5-bisphosphate [( 3H]PIP2) was not altered by prior exposure of adipocytes to insulin. It was possible to see oxytocin-induced breakdown of phosphoinositides but no effect of insulin was seen in intact adipocytes. |
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