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| 八株弯曲菌外膜蛋白的SDS-PAGE分析 | |
| 引用本文： | 刁憬瑜,林志靖,高巽坤,张传彬. 八株弯曲菌外膜蛋白的SDS-PAGE分析[J]. 四川大学学报(医学版), 1991, 0(3) |
| 作者姓名： | 刁憬瑜 林志靖 高巽坤 张传彬 |
| 作者单位： | 华西医科大学微生物学与免疫学教研室(刁憬瑜，林志靖，高巽坤)，华西医科大学微生物学与免疫学教研室(张传彬) |
| 摘 要： | 用超声波处理八株不同来源,不同种和亚种的弯曲菌菌悬液,获得细菌细胞外膜;再用月桂酰肌氨酸钠处理外膜可获得外膜蛋白;最后经SDS-PAGE分离出不同分子量的外膜蛋白。结果表明:各种分子量蛋白质的分布及主要外膜蛋白的数目(2～3条)和分子量大小(33～68kd)均较稳定,提示它们在弯曲菌细胞表面结构中为稳定成分。 |
| 关 键 词： | 空肠弯曲菌 结肠弯曲菌 胎儿弯曲菌 外膜蛋白 十二烷基磺酸钠-聚丙烯酰胺凝胶电泳 |
Analysis of Outer Membrane Proteins From Eight Strains of Campylobacter in SDS-PAGE |
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| Diao Jingyu,Lin Zhijing,Gao Xunkun,Zhang Chuanbin. Analysis of Outer Membrane Proteins From Eight Strains of Campylobacter in SDS-PAGE[J]. Journal of Sichuan University. Medical science edition, 1991, 0(3) | |
| Authors: | Diao Jingyu Lin Zhijing Gao Xunkun Zhang Chuanbin |
| Affiliation: | Diao Jingyu;Lin Zhijing;Gao Xunkun;Zhang Chuanbin Department of Microbiology and Immunology |
| Abstract: | The outer membrane proteins(OMPs) from 8 strains of Campylobacter,including 5 reference strains and 3 localstrains, were isolated and examined bysodium dodecyl sulfate polyacrylamide gelelectrophoresis(SDS-PAGE)The resultsshowed that the population distributionsof molecular weight of OMPs from diffe-rent species and sources were alike basedon the Rank Sum Test analysis. Thenumber of bands of major OMP wasaround 2-3, the molecular weight ofwhich vareid from 33-68kd. Moreover,three C. jejuni local strains isolated frompatients in China showed commoncharacteristics of OMPs to the referencestrains. It is suggested that the OMPs ofCampylobacter are of a conservative andstable structure. |
| Keywords: | Campylobacter jejuni Campylobacter coli Campylobacter fetus Outer membrane proteins SDS-PAGE |
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