结直肠癌中survivin、caspase-3、p21WAF1的蛋白表达及其意义

目的探讨结直肠癌中survivin、caspase-3和p21^WAF1的蛋白表达与临床病理参数的联系以及survivin与caspase-3和p21^WAF1蛋白表达之间的关系。方法采用免疫组织化学SP方法检测15例正常结直肠粘膜和62例结直肠腺癌标本中survivin、caspase-3和p21^WAF1的蛋白表达。结果结直肠腺癌与正常结直肠粘膜比较,survivin、caspase-3和p21^WAF1的蛋白表达差异均有显著性（P〈0.05）。survivin和caspase-3的蛋白表达与淋巴结转移无明显相关（P〉0.05）;而与分化程度均显著相关（P〈0.05）。survivin蛋白与Dukes分期相关（P〈0.05）;但caspase-3的蛋白表达与Dukes分期无关（P〉0.05）。p21^WAF1蛋白表达与分化程度、淋巴结转移和Dukes分期均显著相关（P〈0.05）。survivin蛋白分别与caspase-3、p21^WAF1蛋白表达之间均呈显著负相关（P〈0.01）。结论survivin、caspase-3和p21^WAF1蛋白在结直肠癌的发生和进展中都起着重要的作用。p21^WAF1基因与结直肠癌的恶性进展显著相关,此结论鲜见报道。

Expression of survivin, caspase-3 and p21WAF1 Proteins in Colorectal Ccarcinoma and Their Clinical Significance

Objective To investigate the expression of survivin, caspase-3 and p21WAF1 proteins in colorectal carcinoma and their relationship between their expression and clinical pathology parameter, and correlation between expression of survivin protein and caspase-3, p21WAF1 protein expression respectively. Methods SP immunohistochemical method was used to detect expressions of survivin, caspase-3 and p21WAF1 proteins in 15 normal colorectal mucosa and 62 colorectal carcinoma. Results Colorectal carcinoma compared with normal colorectal mucosa, there was significant differences of survivin, caspase-3 and p21WAF1 protein expression (P<0.05).No correlation was observed between survivin, caspase-3 protein expression and lymph node metastasis (P>0.05), but survivin, caspase-3 protein expression were both correlated with cell differentiation degree (P<0.05). Positive rates of survivin protein was 44.44%(16/36) in A and B of Dukes phase, which lower than positive rates (73.08%) of survivin protein in C and D of Dukes phase(P<0.05). No correlation was observed between caspase-3 protein expression and Dukes phase (P>0.05). There was significant differences between p21WAF1 protein expression and cell differentiation degree, lymph node metastasis, Dukes phase (P<0.05).A highly negative correlation was present between survivin and caspase-3, p21WAF1 protein expression respectively(P<0.01). Conclusion survivin, caspase-3 and p21WAF1 genes may be an important role to formation and progression of colorectal carcinoma. It is rarely reported on relationship between p21WAF1 gene and the malignant progression of colorectal carcinoma.