瘢痕Bcl-2基因与细胞增殖和凋亡的关系

目的:探讨增生性瘢痕成纤维细胞Bcl-2基因表达水平与细胞增殖活性及凋亡程度的关系。方法:用原位杂交、免疫组化染色ABC法,对79例增生性瘢痕组织分别检测Bcl-2mRNA、Bcl-2蛋白和增殖细胞核抗原的表达,并用TUNEL法作原位细胞凋亡检测。结果:75例（94.9％）表达Bcl-2mRNA,70例（88.6％）表达Bcl-2蛋白,两者表达水平呈正相关（rs=0.989,P<0.01）;随成纤维细胞Bcl-2蛋白表达水平升高其增殖活性相应增加,而凋亡相应减少,Bcl-2蛋白+++组与++组（P<0.05）及前两组分别与-组和+组间（P<0.01）两项指标差异均有显著性。结论:增生性瘢痕中成纤维细胞Bcl-2基因高表达可抑制其凋亡,可能由此引起的细胞凋亡减少与其他基因异常所致的细胞过度增殖共同导致瘢痕的形成。

RELATIONSHIP OF BCL- 2 GENE EXPRESSION WITH CELL PROLIFERATION AND APOPTOSIS IN HYPERTROPHIC SCARS

Objective: To study the relationship of Bel -2 gene expression level with cell proliferative activity and apoptosis in hyper-trophic scar. Methods: The expression of Bel - 2 mRNA, Bel - 2 protein and proliferating cell antigen, and the apoptosis in seventy -nine hypertrophic scars were studies using in situ hybridization, insitu cell death detection(TUNEL method) and immunohisto - chem-istry. Results: Of the 79 hypertrophic scars, 75(94. 9% ) and 70(88. 6% ) expressed bcl - 2 mRNA and bcl - 2 protein, respectively. The expression levels of bcl - 2 mRNA and bcl - 2 protein were correlated positively with each other(ra = 0. 989, P < 0. 01) . With the increase in the expression of bcl - 2 protein, the cell proliferating activing increased and apoptosis decreased in hypertrophic scars. There was significant difference of cell proliferation and apoptosis between + + + group and + + group of bcl - 2 protein expression (P < 0.05) as well as between both the former groups, and the negative and + group(P < 0. 01) respectively. Conclusions: Over-expression of bcl - 2 gene inhibts apoptosis of fibroblasts, and the inhibitory intensity increases with the ascending of bcl - 2 gene expression level in fibroblasts. Both the decrease in apoptosis caused by bcl - 2 gene over - expression and the excessive cell proliferation promoted by other gene abnormalities may result in unlimited cell accumulation, which may play an important role in the development and progression of scar.