小牛血清去蛋白提取物联合针刺对脑缺血大鼠治疗作用的观察

目的:探讨小牛血清去蛋白提取物(DCSI)联合针刺治疗脑缺血大鼠的效果是否优于单独应用DCSI或针刺治疗。方法:Wistar大鼠90只,随机分为空白对照组、模型组、针刺组、DCSI组、针刺+DCSI组,每组18只。阻塞大脑中动脉制作脑缺血模型。DCSI给药方法为大鼠尾静脉注射80 mg/kg,1次/d,连续7 d;针刺组针刺"百会"水沟"内关"合谷"太冲"足三里",留针20 min,1次/d,连续7 d。5组分别于造模后1、3、7 d进行神经功能缺损评分,然后6只大鼠制作脑组织切片测定脑梗死体积,6只大鼠测定脑组织含水量,6只大鼠测定脑组织乳酸含量。结果:模型组各指标的测定结果与空白对照组比较均显著增加(P<0.05),说明模型制作成功。各治疗组大鼠的神经功能缺损评分、脑梗死体积、脑组织含水量和乳酸含量均较模型组明显降低(P<0.05);DCSI与针刺联合治疗组与单独应用DCSI或针刺的治疗组比较各项指标降低更为明显(P<0.05)。结论:小牛血清去蛋白提取物联合针刺治疗大鼠脑缺血效果更加显著。

Effect of Combined Application of Deproteinised Calf Serum Injection and Acupuncture on Cerebral Ischemia in Rats

Objective To study the efficacy of combined application of deproteinised calf serum injection (DCSI) and acupuncture in treating cerebral ischemia (CD in rats. Methods Ninety Wistar rats were randomly divided into control, Cl model, Acupunct (acupuncture), DCSI, Acupunct + DCSI groups, with 18 cases in each group. Cl model was duplicated by middle cerebral artery occlusion (MCAO). DCSI (80 mg/kg) was given via the rat's tail vein, once daily for 7 days. Acupuncture was applied to "Baihui" (GV 20), "Shuigou" (GV26), "Neiguan" (PC 6), "Hegu" (LI 4), "Taichong" (LR 3) and "Zusanli" (ST 36), once daily for 7 days. The neurological deficit score (NDS),cerebral infarct volume (CIV), water and lactic acid (LA) contents in cerebral tissues were measured. Results In comparison with control group, NDS, CIV, cerebral water content and LA in model group increased significantly (P<0. 05) s while compared with model group, NDS, CIV, cerebral water content and LA in Acupunct, DCSI and Acupunct + DCSI groups decreased significantly (P<0. 05). Comparison among DCSI group, Acupunct group and DCSI + Acupunct group showed that NDS, CIV, cerebral water and LA contents of Acupunct + DCSI group were significantly lower than those of the former two groups (P<0. 05). No significant differences were found between DCSI and Acupunct groups in the above-mentioned 4 indexes (P>0. 05). Conclusion Combined application of DCSI and acupuncture has a definite effect in improving cerebral ischemia and is superior to that of simple acupuncture or DCSI alone.