乳饮料中5种甜味剂和2种防腐剂的高效液相色谱快速测定法

目的 建立一种适应快速检测乳饮料中乙酰磺胺酸钾、糖精钠、甜蜜素、阿斯巴甜、阿力甜和苯甲酸、山梨酸的高效液相色谱方法.方法 样品经过稀释、沉淀蛋白、高速离心等前处理,用NUCLEODUR C_(18)反相色谱柱分离,磷酸盐缓冲液(调pH=5.0)与乙腈为流动相,进行梯度洗脱,紫外检测波长为205 nm.结果 一次进样分析时长为15 min,5种甜味剂和2种防腐剂均能很好分离,线性范围为5.00～100.0mg/L,相关系数≥0.999 3,RSD为1.5%-4.2%(n=6),平均加标回收率为85.2%～108%.结论 该方法简单快速,重现性和选择性好,是检测乳饮料中甜味剂和防腐剂的有效方法.

Abstract：

Objective To establish a rapid test method for the determination of five sweeteners and two preservativea in the milk beverage by HPLC.Methods Trough a set of pretreatment such as dilution,protein precipitation,hish-speed centrifugation,the samples were tested.With NUCLEODUR C_(18) RP-column separation,phosphate buffer (pH=5.01)and acetonitrile were used as the mobile phase,gradient elution analysis was conducted,the detective wavelength was 205nm.Results The separation of one sample was achieved within 15 min,five sweeteners and two preservatives could be well separated,the linear range was 5.00-100.0 mg/L,r≥0.9993,RSD was 1.5%-4.2%(n=6),the average recovery rate was 85.2%-108%.Condusion This method is simple and quick with better reproducibility and selectivity,it is an effective way to determine sweeteners and preservatiyes in milk beverage.

Rapid Determination of Five Sweeteners and Two Preservatives in Milk Beverage by HPLC

Objective To establish a rapid test method for the determination of five sweeteners and two preservativea in the milk beverage by HPLC.Methods Trough a set of pretreatment such as dilution,protein precipitation,hish-speed centrifugation,the samples were tested.With NUCLEODUR C_(18) RP-column separation,phosphate buffer (pH=5.01)and acetonitrile were used as the mobile phase,gradient elution analysis was conducted,the detective wavelength was 205nm.Results The separation of one sample was achieved within 15 min,five sweeteners and two preservatives could be well separated,the linear range was 5.00-100.0 mg/L,r≥0.9993,RSD was 1.5%-4.2%(n=6),the average recovery rate was 85.2%-108%.Condusion This method is simple and quick with better reproducibility and selectivity,it is an effective way to determine sweeteners and preservatiyes in milk beverage.