双酚A对大鼠睾丸支持细胞的功能影响及机制

目的 探讨双酚A(BPA)对雄性SD大鼠睾丸支持细胞功能的影响及损伤机制.方法 将16～22日龄SPF级雄性SD大鼠的睾丸支持细胞制成3.0×10~6～3.5×10~6个/ml的细胞悬液,设立空白对照组、溶剂对照组和1×10~(-7)、1×10~(-6)、1×10~(-5)、1×10~(-4)mol/L BPA染毒组,观察睾丸支持细胞的增殖、细胞周期以及PCNA、Vimentin蛋白表达的情况.结果 各染毒组睾丸支持细胞的存活率明显低于对照组(P<0.05),G_0/G_1期细胞构成比增加,而S期和M期细胞构成比降低,PI下降.免疫细胞化学结果显示,10~(-4)mol/L、10~(-5) mol/L BPA实验组显著抑制大鼠睾丸支持细胞PCNA的表达.BPA在10~(-4) mol/L、10~(-5)mol/L、10~(-6) mol/L剂量组显著抑制大鼠睾丸支持细胞内Vimentin的表达.结论 双酚A可通过影响睾丸支持细胞的细胞增殖、细胞周期和抑制PCNA、Vimentin蛋白表达而表现出生殖毒性.

Abstract：

Objective To investigate the effects of bisphenol A on SD rat Sertoli cell function and the injury mechanism. Methods SD rat Sertoli cells were treated with bisphenol A at different doses,and the control group,solvent control group were set. Sertoli cell proliferation,cell cycle and PCNA.Vimentin protein expression were observed. Results The survival rate of Sertoli cells in the treated groups was significantly lower than the control group (P<0.05), constitution ratio of G_0/G_1 phase cells increased,while for S phase and M-phase, it decreased,and PI decreased. Immunocytochemistry showed PCNA expression in Sertoli cells was significantly inhibited by 10~(-4),10~(-5) mol/L BPA. Vimentin expression in Sertoli cells was significantly inhibited by 10~(-4), 10~(-5),10~(-6) mol/L BPA. Conclusion Bisphenol A can affect Sertoli cell proliferation,cell cycle and inhibit PCNA.Vimentin protein expression in Sertoli cells.

Effects of Bisphenol A on Rat Sertoli Cell Function and Its Mechanism

Objective To investigate the effects of bisphenol A on SD rat Sertoli cell function and the injury mechanism. Methods SD rat Sertoli cells were treated with bisphenol A at different doses,and the control group,solvent control group were set. Sertoli cell proliferation,cell cycle and PCNA.Vimentin protein expression were observed. Results The survival rate of Sertoli cells in the treated groups was significantly lower than the control group (P<0.05), constitution ratio of G_0/G_1 phase cells increased,while for S phase and M-phase, it decreased,and PI decreased. Immunocytochemistry showed PCNA expression in Sertoli cells was significantly inhibited by 10~(-4),10~(-5) mol/L BPA. Vimentin expression in Sertoli cells was significantly inhibited by 10~(-4), 10~(-5),10~(-6) mol/L BPA. Conclusion Bisphenol A can affect Sertoli cell proliferation,cell cycle and inhibit PCNA.Vimentin protein expression in Sertoli cells.