U937细胞ClqR介导对酵母多糖颗粒的吞噬及其与FcγR的协同作用

在证实了人Ｍφ系Ｕ９３７细胞ＣｌｑＲ在生理离子强度（Ｉ０．１５）下可与１２５Ｉ－Ｃｌｑ结合的基础上研究了ＣｌｑＲ对Ｕ９３７细胞吞噬酵母多糖颗粒（Ｚ）的调节作用。该细胞不吞噬Ｚ，但可吞噬Ｃｌｑ或ＩｑＧ调理的Ｚ（ＣＺ、ＩＺ），若以Ｃｌｑ和ＩｇＧ调理（ＩＣＺ），吞噬指数分别是ＣＺ和ＩＺ的６倍和２．５倍。将调理颗粒热灭活，或加入兔抗人ＣｌｑＦ（ａｂ’）２，或以高浓度Ｃｌｑ或抗人ＣｌｑＲｍＡｂＥ８预处理Ｕ９３７细胞，均可不同程度地抑制对ＣＺ或ＩＣＺ的吞噬，但Ｃｌｑ预处理却使对ＩＺ和Ｚ的吞噬增强。资料表明，Ｕ９３７细胞通过ＣｌｑＲ介导对酵母多糖颗粒的吞噬，且与ＦｃγＲ有协同作用。

The Modulation of Phagocytosis to Zymosan Particles via ClqR and the Association with FcγR by U937 Cells

The modulatory effect of ClqR of U937 cells on the phagocytosis to the zymosan particles(Z) was investigated on the basis of finding that ClqR could combine with 125I-CIq in the physiological ion strength(I0.15) U937 cells were found to ingest the particles opsonized with human Clq or IgG(CZ or IZ) but not Z, and to ingest about 6and 2.5 times more the particles opsonized with both Clq and IgG(ICZ) than CZ and IZ respectively. Specificity of the responses was evidenced by the inhibition of the phagocytosis of CZ and ICZ by F(ab')2 anti-Clq, heat-inactivating of the opsonized particles or the pretreatment of U937 cells with Clq or anti-ClqR mAb E8 and by the enhancement of the phagocytosis of Z and IZ by Clq-pretreted U937 cells. The results indicated that Clq induced receptor-mediated phagocytosis of zymosan particles by U937 cells via ClqR alone or in synergy with FcyR.