五种生长因子mRNA在兔角膜中的表达

目的　了解EGF、TGF-α、bFGF、TGF-β1和PDGF在兔角膜上皮和基质中的表达情况，探讨其在角膜伤口愈合中的作用。方法 应用原位核酸分子杂交方法检测兔角膜上皮和基质中EGF、TGF-α、bFGF、TGF-β1和PDGF mRNA的表达。 结果 EGF、TGF-α和PDGF mRNA仅在正常角膜上皮细胞层表达，基质中未见表达；bFGF和TGF-β1mRNA在正常角膜上皮和基质中均有表达。 结论 角膜能合成和分泌EGF、TGF-α、bFGF、TGF-β1和PDGF,且共同调节着角膜伤口的愈合。

Five Cytokines mRNA Expression in Rabbit Corneal Epithelium and Stroma

Objective To investigate the expression and distribution of epidermal growth factor (EGF), transforming growth factor-α(TGF-α), basic fibroblast growth factor (bFGF), transforming growth factor-β(TGF-β) and platelet-derived growth factor(PDGF) mRNA in the corneal epithelium and stroma of rabbit eyes, and evaluate their effects during corneal wound healing.Methods The expression and distribution of EGF, TGF-α, bFGF, TGF-β1 and PDGF mRNA in the corneal epithelium and stroma of rabbit eyes were detected by in situ hybridization. Results EGF,TGF-α and PDGF mRNA were only expressed in the normal corneal epithelium, but not in the corneal stroma. bFGF and TGF-β1 mRNA were expressed both in the normal corneal epithelium and stroma. Conclusion EGF, TGF-α, bFGF, TGF-β1 and PDGF can be excreted by cornea tissue,and regulate the corneal wound healing procedure.