Emphysema induced by elastase enhances acute inflammatory pulmonary response to intraperitoneal LPS in rats |
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| Authors: | Lídia Maria Carneiro da Fonseca Maycon Moura Reboredo Leda Marília Fonseca Lucinda Thaís Fernanda Fazza Maria Aparecida Esteves Rabelo Adenilson Souza Fonseca Flavia de Paoli Bruno Valle Pinheiro |
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| Affiliation: | 1. Pulmonary Research Laboratory, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil;2. Center of Reproductive Biology, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil;3. Department of Morphology, Institute of Biological Sciences, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil;4. Department of Biophysics and Biometry, Institute of Biology Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Juiz de Fora, Brazil |
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| Abstract: | Abnormalities in lungs caused by emphysema might alter their response to sepsis and the occurrence of acute lung injury (ALI). This study compared the extension of ALI in response to intraperitoneal lipopolysaccharide (LPS) injection in Wistar rats with and without emphysema induced by elastase. Adult male Wistar rats were randomized into four groups: control, emphysema without sepsis, normal lung with sepsis and emphysema with sepsis. Sepsis was induced, and 24 h later the rats were euthanised. The following analysis was performed: blood gas measurements, bronchoalveolar lavage (BAL), lung permeability and histology. Animals that received LPS showed significant increase in a lung injury scoring system, inflammatory cells in bronchoalveolar lavage (BAL) and IL‐6, TNF‐α and CXCL2 mRNA expression in lung tissue. Animals with emphysema and sepsis showed increased alveolocapillary membrane permeability, demonstrated by higher BAL/serum albumin ratio. In conclusion, the presence of emphysema induced by elastase increases the inflammatory response in the lungs to a systemic stimulus, represented in this model by the intraperitoneal injection of LPS. |
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| Keywords: | acute lung injury acute respiratory distress syndrome lipopolysaccharides pancreatic elastase pulmonary emphysema sepsis |
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