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| 旋毛虫成虫期特异性基因的筛选与序列分析 | |
| 引用本文： | 杨静,诸欣平,张新梅,杨雅平,周蕾,高欣,刘明远,Boireau Pascal.旋毛虫成虫期特异性基因的筛选与序列分析[J].中国寄生虫学与寄生虫病杂志,2001,19(2):68-71. |
| 作者姓名： | 杨静 诸欣平 张新梅 杨雅平 周蕾 高欣 刘明远 Boireau Pascal |
| 作者单位： | 1. 首都医科大学寄生虫学教研室 2. 军需大学旋毛虫病实验室 3. Parasitology UnitCNEVALCRV-Maisons-Alfort |
| 基金项目： | 美国中华医学基金会(CMB)资助项目(Parasitology 98-674) |
| 摘 要： | 目的 获得旋毛虫成虫期特异性基因序列。方法 应用旋毛虫成虫期特异性探针对成虫cDNA文库进行杂交筛选、克隆测序及核苷酸序列数据库 (GenBank)查寻比较 ,采用DNAstar软件进行基因序列分析。结果 利用消减杂交 (subtractedhybridization)获得的旋毛虫成虫期特异性探针筛选成虫cDNA文库 ,获得一个长 16 2 9bp的基因。其开放阅读框架由 146 4个核苷酸组成 ,编码 487个氨基酸。序列分析表明 ,该序列是一个尚未见报道的旋毛虫基因序列 ,在 2 0 7～ 2 70位氨基酸间存在两个锌指结构 ,在 5 2～ 6 4,10 8～ 116 ,137～ 16 3,2 2 6～ 2 6 0位氨基酸处存在预测的抗原表位。结论 获得旋毛虫成虫期特异性基因 ,其编码的蛋白具有一定的抗原表位 |
| 关 键 词： | 旋毛虫 成虫cDNA文库 cDNA克隆 序列分析 消减杂交 |
| 文章编号： | 1000-7423(2001)-02-0068-04 |
| 修稿时间： | 2000年5月29日 |
Screening and Sequence Analysis of the Adult Stage-specific Gene of Trichinella spiralis |
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| YANG Jing,ZHU Xin ping,ZHANG Xin mei,YANG Ya ping ZHOU Lei,GAO Xin,LIU Ming yuan,Boireau Pascal.Screening and Sequence Analysis of the Adult Stage-specific Gene of Trichinella spiralis[J].Chinese Journal of Parasitology and Parasitic Diseases,2001,19(2):68-71. | |
| Authors: | YANG Jing ZHU Xin ping ZHANG Xin mei YANG Ya ping ZHOU Lei GAO Xin LIU Ming yuan Boireau Pascal |
| Institution: | Department of Parasitology, Capital University of Medical Sciences, Beijing 100054. |
| Abstract: | OBJECTIVE: To obtain the sequence of the adult stage-specific gene of Trichinella spiralis. METHODS: An adult T. spiralis cDNA library was screened using an adult T. spiralis stage-specific probe. The gene sequence was analyzed by DNAstar software and GenBank database. RESULTS: A cDNA clone of 1,629 bp was obtained by screening the library with an adult stage-specific probe. The clone contained one open reading frame composed of 1,464 bp encoding 487 amino acids. GenBank searching revealed that no identical cDNA was found. Two zinc fingers located at the positions within amino acids 207-270. Some possible antigen epitopes were predicted within the amino acids 52-64, 108-116, 137-163 and 226-260. CONCLUSION: A novel stage-specific gene of adult T. spiralis encoding the protein containing certain predicted antigen epitopes was obtained. |
| Keywords: | Trichinella spiralis adult cDNA library cDNA clone sequence analysis subtracted hybridization |
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