MACC5对大鼠抗Thy1.1系膜增生性肾炎防治作用的研究

目的 :观察中草药有效成分 MACC5对 SD大鼠抗 Thy1.1系膜增生性肾炎实验模型的防治作用 ,探讨其作用机理。方法 :用 SD大鼠胸腺细胞免疫新西兰白兔 ,制备兔抗大鼠胸腺细胞免疫血清 ( ATS) ,再将 ATS经尾静脉注射给SD大鼠 ,诱导其发生系膜增生性肾炎 ( Ms PGN) ,然后腹腔注射 MACC5 ,观察其尿量、尿蛋白定量改变 ,并用 H E染色法检测系膜增殖程度 ,用免疫组化法 ( LSAB)检测其肾小球内细胞增殖核抗原 ( PCNA)的表达。结果 :MACC5两试验组尿量( 2 4小时 )均多于对照组 ( P<0 .0 1) ;MACC5 3.0 m L试验组尿蛋白定量少于对照组 ( P<0 .0 1) ;MACC5两试验组肾小球细胞数均少于对照组 ( P<0 .0 1) ;MACC5两试验组 PCNA的表达均少于对照组 ( P<0 .0 1)。结论 :MACC5对大鼠 Ms PGN实验模型具有利尿、降低尿蛋白和促进肾小球系膜损伤修复的作用。

Study of therapeutic effects of MACC5 on rats with MsPGN induced by anti-Thy1.1

Objective:It is to observe the therapeutic effects of MACC5 which is isolated from traditional Chinese medicine on the rat experimental model with mesangial proliferative glomerulonephritis(MsPGN) induced by anti-Thy1.1,and investigate its mechanism.Methods:Anti-Thy1.1 serum(ATS) was produced by immunizing New Zealand white rabbits with thymocytes of SD rats.SD rat experimental models with MsPGN were induced by intravenously injecting ATS.MACC5 was used to treat expermental models with MsPGN.The urine volumes and protein volumes in urine of rats were observed.The proliferative degree of mesangium was examined by HE stain and the expressions of PCNA in the glomeruli were examined by immunohistochemistry method(LSAB).Results:The urine volumes(24hours) of expermental groups were more than those of control group(P<0.01).The protein volumes in urine of MACC5 3.0mL expermental group were significant lower than those of control group(P<0.01),and the number of intraglomerular cells of experimental groups were marked fewer than those of control group(P<0.01).The expressions of PCNA of experimental groups were significant lower than those of control group(P<0.01).Conclusion:MACC5 has an important role in repairing process of mesangial lesion of MsPGN and has the effects of diureses and decreasing protein volumes in urine.;