槟榔碱诱导口腔角质形成细胞凋亡研究

目的:了解槟榔碱(arecoline)对体外培养的人口腔黏膜角质形成细胞(keratinocyte,KC)凋亡的影响。方法:不同浓度的槟榔碱以及在不同时间处理体外培养的KC,在荧光显微镜下观察KC凋亡形态学改变并计算凋亡百分率,用比色法检测Caspase-3活性的改变。结果:1)槟榔碱能以一定时间和浓度依赖方式诱导培养的KC发生凋亡,其细胞凋亡率较正常对照组明显增高(P<0.05)。2)槟榔碱作用KC,其Caspase-3活性较正常对照组明显增高(P<0.05)。结论:槟榔碱可诱导KC凋亡,Caspase-3可能参与了这一细胞凋亡过程的调控。KC凋亡异常可能是口腔黏膜下纤维性变的重要发病机理之一。

Experimental Study on the Apoptosis of Oral Keratinocytes Induced by Arecoline

Objective:To investigate the apoptotic effect of arecoline on the cultured keratinocytes(KC).Methods:KCs were cultured in vitro.Apoptosis of KCs were induced by arecoline.The changes of apoptotic cells and Caspase-3 activity in KC were evaluated with fluorescence microscopy and colorimeteric assay,respectively,and were compared with normal control group.Results:1)The obvious cell apoptotic change could be observed with fluorescent microscopy after arecoline stimulation.The percentage of apoptosis was significantly higher in a dose and time-dependent manner in the arecoline treated group than that in control group(P<0.05).2)The level of Caspase-3 activity was significantly higher in the arecoline group than normal control group(P<0.05).Conclusion:Arecoline may induce the apoptosis of!KCs,and Caspase-3 may take part in the control of apoptotic process.The apoptosis of KC induced by arecoline may contribute to the pathogenesis of oral submucous fibrosis.