续断高效毛细管电泳指纹图谱研究

目的:利用高效毛细管电泳(HPCE)方法建立中药续断的指纹图谱。方法:采用50μm×65 cm(有效长度57 cm)未涂层石英毛细管柱,以pH 9.20,50 mmol.L-1的硼砂-硼酸为缓冲溶液体系,运行电压25 kV,温度25℃,压力进样5 kPa×30 s,检测波长212 nm。采用"中药色谱指纹图谱相似度评价系统2004A版"软件和聚类分析方法,对样品图谱进行分析。结果:12批续断HPCE指纹图谱共确认了14个共有指纹峰,其中产自湖北的2批药材相似度介于0.8至0.9之间,产自四川的10批药材相似度大于0.9。聚类与相似度结果一致。结论:此法准确、可靠,可为续断药材的质量评价与控制提供科学依据。

Study on high performance capillary electrophoresis fingerprint of Dipsaci Radix

Objective: To investigate the HPCE fingerprint of Dipsaci Radix.Methods: The HPCE fingerprint was established using a 50 μm× 65 cm uncoated fused silica capillary(effective length 57 cm),a buffer comprising 50 mmol·L-1 borate-boric acid at pH 9.20,a running voltage of 25 kV,a capillary temperature of 25 ℃ and a wavelength of 212 nm.The sample was injected under pressure of 5 kPa for 30 s.The HPCE fingerprint was evaluated by the software "Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine Version 2004A" and clustering analysis.Results: By analyzing the HPCE fingerprint,high similarities were found among the 12 samples.The similarity coefficients of the two batches of samples from Hubei were from 0.8 to 0.9,and those of the remaining samples from Sichuan were greater than 0.9.Fourteen major co-possessing peaks were selected as the characteristic fingerprint peaks of Dipsaci Radix.Clustering results were consistent with the similarity analysis.Conclusion: The developed method is accurate and reliable,and the fingerprint analysis can thus be used for the quality assessment and control of Dipsaci Radix.