金银花总黄酮对氧化应激中肝星状细胞的保护作用

目的:研究金银花总黄酮对氧化应激中肝星状细胞(HSC)的保护作用。方法:培养大鼠HSC为模型。实验分为空白对照(DMEM培养基)组、模型(DMEM培养基)组与金银花总黄酮1、2、3、4(12.5、25.0、50.0、100.0μg/ml)组。检测MTT抑制率;测定超氧化物歧化酶(SOD)与乳酸脱氢酶(LDH)活性、丙二醛(MDA)含量、总抗氧化能力(T-AOC)。结果:与空白对照组比较,模型组MTT抑制率降低,MDA含量增加,LDH活性增强,SOD活性减弱,T-AOC减弱,差异有统计学意义(P<0.01或P<0.05);与模型组比较,金银花总黄酮1、2、3、4组MTT抑制率升高,MDA含量减少,LDH活性减弱,SOD活性增强,T-AOC增强,差异有统计学意义(P<0.01或P<0.05)。结论:金银花总黄酮具有抑制肝纤维化形成的作用,其作用机制可能与抑制HSC的增殖及抗氧化应激,以及抑制脂质过氧化反应有关。

Effects of Lonicerajaponica Total Flavonoids on Hepatic Stellate Cell Activation and Lipid Peroxidation in Ox- idative Stress

OBJECTIVE： To study the effect of Lonicera japonica total flavonoids on hepatic stellate cell（HSC） activation and lipid peroxidation in oxidative stress. METHODS： HSC model was induced, and the experiment were divided into blank control group （DMEM culture medium）, model group（DMEM culture medium）, and L. japonica total flavonoids （1）, （2）, （3） and （4） groups （ 12.5,25.0,50.0,100.0μg/ml） The proliferation of HSC was detected by MTT assay. The activity of SOD, and the contents of MDA, T-AOC and LDH were measured. RESULTS： Compared with blank control group, inhibition rate of HSC was decreased in model group, and the activity of SOD and the content of T-AOC were decreased, while the levels of MDA and LDH were in- creased; there was statistical significance （P〈0.01 ,P〈0.05）.Compared with model group, inhibition rate of HSC was increased in L. japonica total flavonoids （1）, （2）, （3） and （4） groups, and the activity of SOD and the content of T-AOC were increased, while the levels of MDA and LDH were decreased; there was statistical significance （P〈 0.01,P〈 0.05）. CONCLUSIONS： L. japonica to- tal flavonoids can prevent the formation of hepatic fibrosis probably by inhibiting HSC proliferation, oxidative stress and lipid peroxidation.