聚酰胺树脂精制裸花紫珠总黄酮的工艺优选

目的：优选聚酰胺树脂分离纯化裸花紫珠总黄酮的工艺条件。方法：以总黄酮含量为指标，利用静态-动态吸附-洗脱试验考察聚酰胺树脂纯化总黄酮的行为，通过单因素试验考察饱和吸附量、pH、洗脱溶剂用量等工艺参数。结果：最佳精制工艺参数为上样液质量浓度0.073 g·mL^-1，上样量2 BV，上样液pH 3.0，加水3 BV洗脱杂质，用80%乙醇3 BV洗脱，收集乙醇洗脱液；总黄酮纯度由5.6%提高至42.3%，平均保留率89.6%（RSD 3.56%）。结论：采用聚酰胺树脂纯化裸花紫珠总黄酮的效果良好，为裸花紫珠新制剂的开发提供实验依据。

Optimization of Purification Technology for Total Flavonoids from Callicarpa nudiflora by Polyamide Resin

Objective: To optimize purification technology of total flavonoids from Callicarpa nudiflora by polyamide resin. Method: With the content of total flavonoids as index,adsorption and elution behavior of polyamide resin for total flavonoids was investigated by stastic-dynamic adsorption-elution test,single factor tests were adopted to optimize purification technology for total flavonoids taking saturated adsorption,pH,elution volume and other parameters. Result: Optimum purification technology parameters were as follows:the concentration of sample solution 0.073 g·mL^-1,sample volume of 2 BV,pH of sample solution 3.0,eluted impurities with 3 BV water,then eluted with 3 BV of 80% ethanol;Purity of total flavonoids was up to 42.3% from 5.6%,average retention rate was 89.6%(RSD 3.56%). Conclusion: Purification effect of total flavonoids from C. nudiflora by polyamide resin was feasible,and this study could provide experimental basis for development of new formulations from C. nudiflora.