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| 二氯二苯二氯乙烯的类雌激素活性的实验研究 | |
| 引用本文： | 张明,董力. 二氯二苯二氯乙烯的类雌激素活性的实验研究[J]. 环境与健康杂志, 2008, 25(3): 236-237 |
| 作者姓名： | 张明 董力 |
| 作者单位： | 中国疾病预防控制中心环境与健康相关产品安全所毒理室,北京,100050 |
| 基金项目： | 中国疾病预防控制中心环境与健康相关产品安全所基金资助项目 |
| 摘 要： | 目的研究二氯二苯二氯乙烯(DDE)的类雌激素活性对人乳腺癌细胞株MCF-7增殖的影响。方法采用了体外MCF-7细胞增殖试验检测了DDE(3×10-5、3×10-6、3×10-7、3×10-8、3×10-9 mol/L)的类雌激素活性,并用生长曲线分析对其作用机制进行了初步探讨。结果3×10-6 mol/LDDE染毒组吸光度值高于溶剂对照组且MST-7细胞增殖的生长曲线与10-9 mol/L17β-雌二醇染毒组相似。结论DDE具有刺激MCF-7细胞增殖的类雌激素活性,其机制可能与17β-雌二醇相同。 |
| 关 键 词： | 农药 环境雌激素 细胞增殖 |
| 文章编号： | 1001-5914(2008)03-0236-02 |
| 修稿时间： | 2007-10-18 |
Evaluation of Estrogenic Activity of DDE by MCF-7 Cell Proliferation Bioassay |
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| ZHANG Ming,DONG Li. Evaluation of Estrogenic Activity of DDE by MCF-7 Cell Proliferation Bioassay[J]. Journal of Environment and Health, 2008, 25(3): 236-237 | |
| Authors: | ZHANG Ming DONG Li |
| Abstract: | Objective To evaluate the estrogen activity of DDE by using MCF-7 cell proliferation bioassay.Methods The estrogen activity of DDE was tested by using the MCF-7 cell multiplication experiment in vitro and the mechanism was explored preliminarily through growth curve analysis.Results DDE showed an obvious estrogenic activity in the MCF-7 cell proliferation and the cell cycle analysis indicated that cell proliferation index in DDE group was significantly higher than that in the control group.Conclusion DDE has the estrogenic activity and the mechanism may be the same as that of E2,that is,binding with the estrogen receptor is the first step of the following biological effects. |
| Keywords: | Pesticides Environment estrogen Cell multiplication |
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