| 小鼠牙本质涎磷蛋白基因启动子的克隆与序列分析 |
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| 引用本文： | 何文喜,牛忠英,赵守亮,金卫林,高杰. 小鼠牙本质涎磷蛋白基因启动子的克隆与序列分析[J]. 现代口腔医学杂志, 2005, 19(2): 183-185 |
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| 作者姓名： | 何文喜 牛忠英 赵守亮 金卫林 高杰 |
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| 作者单位： | 1. 710032,西安,第四军大学口腔医学院鼢
2. 解放军306医院 |
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| 基金项目： | 国家自然科学基金资助项目(编号:30200315) |
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| 摘 要： | 目的 克隆小鼠牙本质涎磷蛋白(dentin sialophosphoprotein，DSPP)基因启动子片段。方法 培养MDPC一23细胞，从培养的细胞中提取基因组DNA，利用设计的上下游引物，进行PCR反应。将扩增得到的DSPP基因启动子片段克隆到pMD18-T栽体，酶切鉴定后，进一步进行DNA序列测定。结果 酶切结果表明成功构建重组质粒，序列分析结果与国外报道一致。结论 成功克隆获得小鼠DSPP基因的启动子片段。
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| 关 键 词： | 基因启动子 小鼠 牙本质涎磷蛋白 克隆 培养的细胞 序列分析 重组质粒 酶切 PCR反应 片段 |
cDNA cloning and sequencing of DSPP promoter from MDPC-23 cells |
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| HE Wenxi,NIU Zhongying,ZHAO Shouliang,et al.. cDNA cloning and sequencing of DSPP promoter from MDPC-23 cells[J]. Journal of Modern Stomatology, 2005, 19(2): 183-185 |
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| Authors: | HE Wenxi NIU Zhongying ZHAO Shouliang et al. |
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| Affiliation: | HE Wenxi,NIU Zhongying,ZHAO Shouliang,et al. College of Stomatology,The Fourth Military Medical University,Xi'an 710032 |
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| Abstract: | Objective To clone DSPP promoter from mouse odontoblast cell line MDPC-23.Methods Mouse genomic DNA was isolated from cultured MDPC-23 cells.The desired DNA prduct was generated by PCR with a set of synthesized primers.The amplified segment was cloned into pMD18-T vector and confirmed by restriction endonuclease mapping and DNA sequencing.Results The recombinant plasmid was verified by the restriction endonuclease map,and the result of partial sequencing demonstrated that the sequence of DSPP promoter was consistent with those presented in GeneBank.Conclusion The promoter of DSPP gene was obtained successfully from MDPC-23 cells. |
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| Keywords: | MDPC-23 Dentin sialophosphoprotein Clone Sequencing |
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