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| 应用毛细管血红蛋白电泳技术筛查血红蛋白Constant Spring | |
| 引用本文： | 谢杏梅,周剑英,李坚. 应用毛细管血红蛋白电泳技术筛查血红蛋白Constant Spring[J]. 中国优生与遗传杂志, 2012, 0(11): 30-30,129,F0002 |
| 作者姓名： | 谢杏梅 周剑英 李坚 |
| 作者单位： | 广州市妇女儿童医疗中心产前诊断中心生化遗传室,广州510623 |
| 摘 要： | 目的建立一种在人群中筛查血红蛋白Constant Spring(Hb CS)的简单、经济的实验室方法。方法采用法国Sebia公司Capillarys 2全自动毛细管电泳仪及配套试剂进行血红蛋白电泳,检测Hb CS。阳性标本用分子检测技术验证。结果在23 842例临床标本中,共检出Hb CS携带者71(0.3%)例;Hb CS杂合子携带者血中Hb CS浓度为0.6%(0.1-1.0%)。结论本研究首次报道了本地区人群的Hb CS频率,表明毛细血管电泳仪可用于地中海贫血高发地区的人群筛查。 |
| 关 键 词： | 血红蛋白Constant Spring 毛细管电泳 筛查 |
Screening for hemoglobin Constant Spring using the Sebia capillary electrophoresis system. |
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| XIE Xing - mei,ZHOU Jian - ying,LI Jian. Screening for hemoglobin Constant Spring using the Sebia capillary electrophoresis system.[J]. Chinese Journal of Birth Health & Heredity, 2012, 0(11): 30-30,129,F0002 | |
| Authors: | XIE Xing - mei ZHOU Jian - ying LI Jian |
| Affiliation: | . ( Biochemical Laboratory, Prenatal Diagnostic Center, Guangzhou Women ＆ Children Medical Center, Guangzhou 510623, China. ) |
| Abstract: | Objective： To develop a rapid, economical and efficient approach for screening for hemoglobin Constant Spring （ Hb CS）. Methods： The Sebia capillarys 2 was used to analysis hemoglobin and detect the Hb CS variant. For the samples suspected of Hh CS, reverse dot -blot was used for confirming. Results： In a cohort of 23 842 individuals, Hb CS was detected in 71 （0. 3% ）. The lev- els of Hb CS in heterozygotes ranged from 0. 1 - 1.0% with an average of 0. 6%. Conclusion： It was proposed the reported 0. 3% as a re- alistic figure for the prevalence of Hb CS in South China. The Sebia Capillarys 2 is a suitable device for the identification and Hb CS. |
| Keywords: | Hemoglobin Constant Spring Capillary electrophoresis Screening |
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