高效液相色谱法同时测定脑络欣通复方提取物中三七皂苷和人参皂苷含量

目的建立同时测定中药复方脑络欣通提取物中三七皂苷R1,人参皂苷Rb1、Rg1、Rd、Re的高效液相色谱法分析方法,为脑络欣通制剂的质量控制提供依据。方法采用Welch Materials C8色谱柱(4.6mm×250mm,5μm),选择乙腈-水作为流动相,进行梯度洗脱,流速1.2ml/min,检测波长为203nm,柱温20℃。结果三七皂苷R1,人参皂苷Rb1、Rg1、Rd、Re分别在0.081 7~0.980 4(r=0.999 9)、0.315 7~3.788 4(r=0.999 9)、0.300 0~3.600 0(r=0.999 6)、0.077 7~0.932 4(r=0.999 9)、0.074 6~0.895 2(r=0.999 9)μg与峰面积呈良好的线性关系;平均回收率分别为99.72%、99.65%、101.23%、99.38%、100.62%(n=6)。结论所建立的高效液相色谱方法简单方便、准确可靠,5种物质分离度佳、重复性好,可用于中药复方脑络欣通的质量控制。

Simultaneous Determination of Notoginsenoside and Ginsenosides in Compound Naoluoxintong Extracts by High-performance Liquid Chromatography

Objective To establish a high performance liquid chromatography （HPLC） method for simultaneous determination of notoginsenoside R1 and ginsenosides Rb1,Rg1,Rd,and Re in compound Naoluoxintong extracts and to provide a methodological basis for the quality control of compound Naoluoxintong.Methods HPLC was performed on an Welch Materials C8 column （4.6 mm × 250 mm,5 μm） with a mobile phase of acetonitrile water for gradient elution at a flow rate of 1.2 ml/min,a detection wavelength of 203 nm,and a column temperature of 20 ℃.Results The content of notoginsenoside R1 and ginsenosides Rb1,Rg1,Rd,and Re showed a good linear relationship with the peak area within 0.081 7-0.980 4 μg （r =0.999 9）,0.315 7-3.788 4 μg （r=0.999 9）,0.300 0-3.600 0μg （r=0.999 6）,0.077 7-0.932 4 μg （r=0.999 9）,and 0.074 6-0.895 2μg （r=0.999 9） ; the average recovery rates were 99.72％,99.65％,101.23％,99.38％,and 100.62％ （n=6）.Conclusion The established HPLC method is simple,convenient,accurate,reliable,and repeatable.With this method,notoginsenoside R1 and ginsenosides Rb1,Rg1,Rd,and Re can be well separated,and this method can be used for the quality control of compound Naoluoxintong.