Human cytomegalovirus. Assay by counting infected cells |
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| Affiliation: | 1. Department of Pediatrics, University of Southern California School of Medicine USA;2. the Childrens Hospital of Los Angeles USA;1. University College London Hospital, London, United Kingdom;2. London School of Hygiene and Tropical Medicine, London, United Kingdom;1. Division of Virology, ICMR-National Institute of Cholera and Enteric Diseases, Beliaghata, Kolkata, West Bengal, India;2. Regional Virus Research and Diagnostic Laboratory, ICMR‐National Institute of Cholera and Enteric Diseases, Beliaghata, Kolkata, West Bengal, India;1. Division of Epidemiology, Indian Council of Medical Research, National Institute of Cholera and Enteric Diseases, Kolkata, India;2. Glocal Hospital, Krishnanagore, West Bengal, India;3. Division of Bacteriology, Indian Council of Medical Research, National Institute of Cholera and Enteric Diseases, India;3. Department of Microbiology, Faculty of Medicine and Health Sciences, SGT University, Gurugram, Haryana, India;1. Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, India;2. Department of Microbiology, Dr. Baba Saheb Ambedkar Medical College and Hospital, New Delhi, India;1. Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran;2. Department of Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran;3. Department of Pediatrics, University Medical Center Utrecht, Utrecht, The Netherlands;4. Department of Immunology, University Medical Center Utrecht, Utrecht, The Netherlands |
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| Abstract: | An assay method for human cytomegalovirus is described that is based on counts of infected embryonic human skin and muscle fibroblastic cells on coverslips inoculated with 0.1 ml of diluted virus suspension. Counts made 48 hours after inoculation, when the preparations are fixed and stained, include all initially infected cells. Counts of infected cells are linearly proportional to the inoculum dilution. There is no significant loss of infected cells from the coverslips during preparation, and infected cells are randomly distributed on the coverslips. |
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