Deoxyribonucleic acid synthesis in vaccinia virus-infected HeLa cells |
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| Affiliation: | 1. Laboratory of Aquatic Biology, KU Leuven, campus Kulak Kortrijk, E. Sabbelaan 53, 8500 Kortrijk, Belgium;2. Proteomic and Microbiology Department, University of Mons, Avenue du champ de Mars 6, 7000 Mons, Belgium;3. Analytical and Circular Chemistry (ACC), Institute for Materials Research, Hasselt University, Martelarenlaan 42, 3500 Hasselt, Belgium;4. Sustainable Materials Lab, Department of Chemical Engineering, KU Leuven, campus Kulak Kortrijk, 8500 Kortrijk, Belgium;5. Lab of Molecular Plant Biology, Kasteelpark Arenberg 31, 3001 Leuven, Belgium;6. KU Leuven Plant Institute, Kasteelpark Arenberg 31, 3001 Leuven, Belgium;7. Wyatt Technology Europe GmbH, DE-56307 Dernbach, Germany |
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| Abstract: | The control of DNA synthesis by the addition of graded concentrations of thymidine to HeLa cell cultures inhibited by 5-fluorodeoxyuridine has been determined to be a regulation at the cellular level and not a population selection process. In infected cells the rate of of vaccinia virus DNA in the presence of FUDR is not a function of the thymidine concentration. Instead, suboptimal thymidine levels were utilized preferentially to support optimal viral DNA synthetic rates which ceased abruptly when depletion of thymidine occurred. When FUDR was added early in the infectious cycle, the reduced yield of infectious virus obtained was also formed at a maximal rate. |
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