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高效液相色谱法测定垂盆草胶囊中槲皮素含量
引用本文:杨帆,邓开英. 高效液相色谱法测定垂盆草胶囊中槲皮素含量[J]. 中国药业, 2007, 16(3): 17-18
作者姓名:杨帆  邓开英
作者单位:重庆市药品检验所,重庆,401121
摘    要:目的建立垂盆草胶囊中槲皮素的含量测定方法。方法采用高效液相色谱法,色谱柱为C18柱(250mm×4.6mm,5μm),流动相为甲醇-0.4%磷酸溶液(50:50),检测波长为370nm。结粜槲皮素进样量在0.04152-0.4152μg范围内与峰面积具有起好的线性关系(r=0.9999),平均回收率为101.9%。RSD为2.5%(n=6)。结论该法简单、快速.可为垂盆草胶囊的质量控制提供依据。

关 键 词:垂盆草胶囊  槲皮素  高效液相色谱法  含量测定
文章编号:1006-4931(2007)03-0017-02
收稿时间:2006-11-30
修稿时间:2006-11-30

Determination of Quercetin in Chuipencao Capsule by HPLC
Yang Fan,Deng Kaiying. Determination of Quercetin in Chuipencao Capsule by HPLC[J]. China Pharmaceuticals, 2007, 16(3): 17-18
Authors:Yang Fan  Deng Kaiying
Affiliation:Yang Fan, Deng Kaiying, ( Chongqing Institute for Drug Control, Ghongqing, China 401121)
Abstract:Objective To establish a method for the determination of quercetin in Chuipencao capsule. Methods HPLC method was adopted and a C18 column (250mm ×4. 6mm,5μm) was used. The mobil phase consisted of methanol-0. 4% phosphoric acid (50:50).The detection wavelength was at 370 nm. Results The calibration curve of quercetin was linear in the range of 0.041 52-0. 415 2 μg( r =0.999 9), the average recovery rate was 101.9%, RSD=2.5%(n=6).Concluslon The method is simple, quick, and can be used for quality control of Chuipencao capsule.
Keywords:Chuipencao capsule   quercetin   HPLC   content determination
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