| 大鼠局灶性脑缺血/再灌注后血脑屏障通透性改变时相的研究 |
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| 引用本文： | 杨莹,史秀丽,何玮,傅佳.大鼠局灶性脑缺血/再灌注后血脑屏障通透性改变时相的研究[J].中风与神经疾病杂志,2010,27(2). |
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| 作者姓名： | 杨莹 史秀丽 何玮 傅佳 |
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| 作者单位： | 安徽医科大学第一附属医院神经内科,安徽合肥,230022 |
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| 基金项目： | 安徽省教育厅自然科学基金(No.2005KJ250) |
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| 摘 要： | 目的探讨大鼠局灶性脑缺血/再灌注(I/R)后氧自由基(OFR)与血脑屏障(BBB)通透性改变的时相关系。方法成年健康SD大鼠随机分为脑缺血/再灌注组、正常组及假手术组,分别在脑缺血2h后再灌注各时间点观察脑组织EB含量,血清超氧化物酶(SOD)活力、丙二醛(MDA)含量以及脑组织基质金属蛋白酶2(MMP-2)的表达。结果OFR与脑组织EB含量有相同时相变化趋势,参与BBB通透性增高的整个过程,同期伴随MMP-2表达,且OFR峰值期与BBB通透性增高的第一高峰时相符合。结论OFR是导致BBB通透性增高的重要因素,尤其在其通透性增高的早期发挥重要作用,MMP-2激活可能是OFR损伤BBB的机制之一。
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| 关 键 词： | 脑缺血再灌注损伤 血脑屏障 氧自由基 基质金属蛋白酶2 |
Time-phase study on the permeable changes of blood brain barrier after focal cerebral ischemia/reperfusion(I/R) in rats |
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| YANG Ying,SHI Xiu-li,HE Wei,et al..Time-phase study on the permeable changes of blood brain barrier after focal cerebral ischemia/reperfusion(I/R) in rats[J].Journal of Apoplexy and Nervous Diseases,2010,27(2). |
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| Authors: | YANG Ying SHI Xiu-li HE Wei |
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| Institution: | YANG Ying,SHI Xiu-li,HE Wei,et al.(Department of Neurology,The First Affiliated Hospital of Anhui Medical University,Hefei 230022,China) |
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| Abstract: | Objective The time-phase relationship between oxygen free radical(OFR) and the blood brain barrier(BBB) permeability was observed.Methods Healthy SD rats were randomly divided into the control group,the sham group and the I/R group.The activity of SOD,the content of MDA and the expression of MMP-2 at each time point of reperfusion after 2h cerebral ischemia were observed.Results The content of OFR and EB had the same time-phase variability,while the expression of MMP-2 happened simultaneously.What's more,th... |
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| Keywords: | Brain ischemia/Reperfusion injury Blood brain barrier Oxygen free radical Matrix metalloproteinase-2 |
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