湖北省手足口病肠道病毒71型和柯萨奇病毒A组16型VP1基因特征

目的 了解湖北省手足口病肠道病毒71型（EV71）和柯萨奇病毒A组16型（Cox A16）的VP1基因特点，研究该省EV71和Cox A16的基因型别及分子进化特征。方法 对2014-2015年湖北省26株EV71和27株Cox A16流行株的VP1基因测序，分析其同源性和遗传进化特征。结果 2014-2015年湖北省EV71和Cox A16流行株VP1核苷酸和蛋白氨基酸序列同源性均较高，EV71流行株的蛋白氨基酸和核苷酸序列同源性分别为97.6%~100.0%和92.3%~100.0%。Cox A16流行株的蛋白氨基酸和核苷酸序列同源性分别为96.6%~100.0%和92.3%~100.0%。EV71的VP1基因系统进化分析表明，湖北省流行的EV71与我国其他地区流行的EV71均位于一个大的分支上，均为C4基因型的C4a亚型。Cox A16流行株与B1b亚型代表株位于同一分支上，主要为B1b亚型。结论 2014-2015年湖北省EV71和Cox A16流行株分别为C4a亚型和B1b亚型，其核苷酸和氨基酸与国内外其他地区的手足口病病毒株均有较高同源性。

Analysis on genetic characteristics of VP1 genes of enterovirus 71 and coxsackievirus A16 in Hubei,2014-2015

Objective To understand the genetic characteristics of Enterovirus 71 (EV71) and Coxsackievirus A16 (Cox A16) causing hand foot and mouth disease in Hubei province.Methods The genotype and molecular characteristics of VP1 genes of EV71 and Cox A16 strains circulating in Hubei from 2014 to 2015 were analyzed.Results The EV71 strains shared 92,3％-100.0％ and 97.6％-100.0％ homology respectively in nucleotide and amino acid sequences of VP1 gens.The Cox A16 strains shared 92.3％-100.0％ and 96.6％-100.0％ homology respectively in nucleotide and amino acid sequences of VP1 genes.Phylogenetic analysis indicated that the EV71 strains belonged to sub genotype C4a and the Cox A16 strains belonged to sub genotype B1b.Conclusion The genotypes of EV71 and Cox A16 strains in Hubei from 2014 to 2015 were C4a and B1b respectively.The strains'nucleotide and amino acid shared high homology with the strains isolated from other areas both at home and abroad.