增生性瘢痕和瘢痕疙瘩组织中TGF-β1及Ⅰ、Ⅲ型胶原基因表达的改变及意义

目的检测增生性瘢痕（Ｈ）和瘢痕疙瘩（Ｋ）组织中ＴＧＦβ１及Ⅰ、Ⅲ型前胶原ｍＲＮＡ的表达，了解其相互关系及意义。方法斑点杂交分析检测Ｈ和Ｋ组织中Ⅰ、Ⅲ前胶原及ＴＧＦβ１ｍＲＮＡ稳态水平的改变；原位杂交检测ＴＧＦβ１ｍＲＮＡ在组织中的空间分布。结果①Ｋ和Ｈ组织中ＴＧＦβ１ｍＲＮＡ稳态水平明显高于Ｎ组和Ｓ组；②Ｋ选择性Ⅰ型前胶原ｍＲＮＡ表达增强，而Ｈ组织中Ⅰ、Ⅲ前胶原ｍＲＮＡ表达均增强。结论ＴＧＦβ１在Ｈ和Ｋ的发病机理中起了重要作用，Ｋ和Ｈ发生发展中具有不同的分子机理。

Changes in TGF1 and type , procollagen gene expression in keloid and hypertrophic scar

Objective The expression of mRNA for TGF1,type , procollagen in keloid and hypertrophic scar was investigated in order to elucidate the pathogenesis of and the difference between keloid and hypertrophic scar. Method Dotblot hybridization analysis was used to examine the type , procollagen and the expression levels of steadystate mRNA.In situ hybridization allowed direct assessment of the distribution of TGF1 mRNA in the tissue. Results Our study indicated that the expression level of mRNA for TGF1 significantly increased in keloid and hypertrophic scar compared with normal scar and normal skin.In keloid tissues,type precollagen mRNA expression was selectively increased.However,in hypertrophic scar,type and collagen mRNAs expressions were simultaneously increased.WTHZConclusion TGF1 plays an important role in the pathogenesis of keloid and hypertrophic scarring.It implicates that distinct molecular mechanisms are operative in the development of keloid and hypertrophic scarring.