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| 肝癌细胞中IL-18表达及其调控机制的研究 | |
| 引用本文： | 杨贤子,程甜甜,骆志国,蒋飞,柴海霞. 肝癌细胞中IL-18表达及其调控机制的研究[J]. 实用癌症杂志, 2011, 26(5): 456-458 |
| 作者姓名： | 杨贤子 程甜甜 骆志国 蒋飞 柴海霞 |
| 作者单位： | 湖北省十堰市湖北医药学院附属太和医院,442000 |
| 基金项目： | 十堰市太和医院院级基金项目 |
| 摘 要： | 目的探讨肝癌细胞调节IL-18生物学功能的主要分子机制。方法体外培养正常人类肝细胞株QSG-7701和原发性肝癌细胞株（MHCC97-L和HCCLM3）,采用RT-PCR法,分别检测这3种细胞株中IL-18及其相关正负调节基因mRNA的表达变化。结果与2种原发性肝癌细胞株比较,正常人类肝细胞株中IL-18及其正调节基因粒细胞蛋白水解酶3（proteinase-3）mRNA表达量较高,但正调节基因caspase-1和负调节基因caspase-3 mRNA表达水平无统计学差异。结论原发性肝癌细胞中IL-18 mRNA表达减弱,可能与proteinase-3表达失衡有关。 |
| 关 键 词： | 肝癌 白介素18 IL-1β转化酶 粒细胞蛋白水解酶3 |
Expression of Interleukin-18 and Interleukin-18 Regulatory Genes in Human Hepatocellular Carcinoma Cells and Normal Liver Cells |
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| YANG Xian-zi,CHENG Tian-tian,LUO Zhi-guo,et al.. Expression of Interleukin-18 and Interleukin-18 Regulatory Genes in Human Hepatocellular Carcinoma Cells and Normal Liver Cells[J]. The Practical Journal of Cancer, 2011, 26(5): 456-458 | |
| Authors: | YANG Xian-zi CHENG Tian-tian LUO Zhi-guo et al. |
| Affiliation: | YANG Xian-zi,CHENG Tian-tian,LUO Zhi-guo,et al.Department of Oncology,Taihe Hospital,Hubei University of Medical,Shiyan,442000 |
| Abstract: | Objective This study aimed to investigate the expression of IL-18 and IL-18 regulatory genes in human hepatocellular carcinoma cells and normal liver cells,than illuminate the possible molecular mechanism of the regulation of IL-18 in human hepatocellular carcinoma cells.Methods The normal human liver cell line QSG-7701 surrounding tissue and the human hepatocellular carcinoma cell lines MHCC97-L and HCCLM3 were cultured till the exponential phase of growth.RT-PCR was used to detect mRNA expression of IL-18... |
| Keywords: | Hepatocellular carcinoma Interleukin-18 Caspase-1 Proteinase-3 |
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