安宫牛黄丸（含天然麝香或人工麝香）对实验性脑缺血的保护作用

目的:研究比较含人工麝香或天然麝香的安宫牛黄丸对实验性脑缺血的保护作用.方法:采用大脑中动脉栓塞(MCAT)与大脑中动脉缺血再灌注(MCAO)两种动物模型,观察两种安宫牛黄丸(84～333 mg· kg-1)对大鼠的神经症状、脑梗死范围以及对缺血侧脑组织酸中毒和自由基代谢的影响.结果:安宫牛黄丸(含人工麝香或天然麝香)167,333 mg· kg-1剂量组可显著改善MCAT大鼠的神经症状；安宫牛黄丸(含天然麝香)84,167 mg· kg-1剂量组可分别减少MCAT大鼠脑梗死范围27％和37％,安宫牛黄丸(含人工麝香)84～333 mg·kg-1显著减少MCAT大鼠脑梗死灶面积21％～32％,两种安宫牛黄丸对应各剂量组间无显著性差异；两者均可显著降低MCAO大鼠的LDH(10％～12％)和MDA(28％～38％)含量,并显著升高SOD活力(15％～36％)和GSH含量(14％～36％).结论:含人工麝香或天然麝香的安宫牛黄丸均可通过改善氧化应激损伤而对实验性脑缺血有保护作用,且二者作用未见显著差异.

Protective Effect of An-Gong-Niu-Huang-Wan (Containing Natural or Artificial Moschus) on Experimental Cerebral Ischemia in Rats

This article was aimed to investigate the protective effects of An-Gong-Niu-Huang-Wan containing artificial moschus or natural moschus on experimental cerebral ischemic model rats. Cerebral ischemia was established by middle cerebral artery thrombosis (MCAT) or middle cerebral artery occlusion (MCAO) in rats. The neurological function, cerebral infarct volume, acidosis and free radical metabolism were observed in the MCAT or MCAO rats after intragastric administration with An-Gong-Niu-Huang-Wan containing artificial or natural moschus at the dosage of 84~333 mg/kg. The neurological function and the infarct region was significantly ameliorated after treated with two kinds of An-Gong-Niu-Huang-Wan at the dosage of 84~333 mg/kg, when compared with non-treated MCAT rats. The lactate dehydrogenase (LDH) activity and malondialdehyde (MDA) content were significantly decreased by 10%~12% and 28%~38% respectively, and superoxide dismutase (SOD) activity and glutathiose (GSH) content were significantly increased by 15%~36% and 14%~36% respectively in MCAO rats after intragastric administration with An-Gong-Niu-Huang-Wan containing artificial or natural moschus, when compared with non-treated MCAO rats. It showed no statistical significant difference when compare the effect of two kinds of An-Gong-Niu-Huang-Wan. It was concluded that An-Gong-Niu-Huang-Wan with either artificial or natural moschus treatment could attenuate experimental cerebral ischemic rats. And there were no significant differences on the effects of both types.