孕激素对绒癌细胞系Galectin-3表达的影响

目的通过体外试验研究孕激素对BeWo绒癌细胞系中Galectin-3蛋白的表达,探讨其对滋养细胞侵袭性相关基因表达的影响作用。方法体外培养BeWo绒癌细胞,分别加入不同浓度的孕激素24h后,采用免疫印迹技术、免疫荧光技术测定各种条件下的Galectin-3蛋白的表达。结果Bewo绒癌细胞系表达Galectin-3。分别用0、10-7、10-6、10-5mol/L孕激素处理24h后,10-7、10-6mol/L的孕激素可以显著提高BeWo绒癌细胞系中Galectin-3蛋白的表达(P<0.05,相反10-5mol/LP使Galectin-3表达降低(P<0.05)。结论(1)BeWo绒癌细胞系有Galectin-3的表达。(2)低浓度的孕激素可以诱导Galectin-3蛋白的表达。(3)高浓度的孕激素对Galectin-3蛋白的表达有下调作用。

Effect of progestogen on galectin-3 expression in choriocarcinoma cell line

Objective:To show the effect of progesterone on the invasiveness-associated gene expression in choriocarcinoma cells.The effect of P on Galectin-3 expression in BeWo choriocarcinonla cells line was detected in vitro.Methods:BeWo choriocarcinoma cells were incubated with progesterone(0 M,10 -7 M,10 -6 M,10 -5 M)for 24 hours,respectively.The expression of Galectin-3 protein detected by in immunofluorescence and western bloting.Results: Galectin-3 protein expression in BeWo cells incubated with 10 -7 M,10 -6 M progesterone increased(P<0.05).However 10 -5 M progesterone could significantly decrease the expression of Galectin-3 protein(P<0.05).Conclusion:(1)There was expression of Galectin-3 protein in BeWo choriocarcinoma cells.(2)Low density progesterone could elevate the level of Galectin-3 protein.(3)High density progesterone could down-regulate the expression of Galectin-3 protein in BeWo choriocarcinoma cells.