青蒿琥酯对小鼠玫瑰痤疮样炎症的作用观察

目的 观察青蒿琥酯对小鼠玫瑰痤疮样炎症的影响.方法 在25只7周龄BALB/c雄鼠背部皮下注射40μl抗菌肽LL-37,每12小时1次,共4次,制备玫瑰痤疮样小鼠模型,并将其随机等分为5组,每次注射LL-37后模型组给予生理氯化钠溶液灌胃,治疗组分别给予25、50、100 mg/kg青蒿琥酯溶液灌胃,阳性对照组给予30 mg/kg盐酸多西环素溶液灌胃;另取5只小鼠作为空白对照组,仅背部皮下注射4次纯水.首次注射LL-37后48 h观察各组皮损和红斑评分,取背部注射部位皮肤行HE染色,观察组织结构并计数炎症细胞数量,ELISA法检测皮损髓过氧化物酶(MPO)活性.结果 模型组皮损炎症反应明显,红斑评分(3.20±0.84)、炎症细胞计数(517.27±99.43)和MPO活性(0.57±0.08)均显著高于空白对照组(均P＜0.01),阳性对照组红斑评分(1.60±0.89)、炎症细胞计数(270.93±124.63)和MPO活性(0.40±0.05)均显著低于模型组,差异有统计学意义(P＜0.05、0.01、0.01).50和100 mg/kg青蒿琥酯组红斑评分分别是1.80±0.84和1.40±0.55,显著低于模型组(P＜0.05、0.01),炎症细胞计数分别为286.00±33.72和258.00±36.44,显著低于模型组(均P＜0.01),MPO活性分别是0.43±0.05和0.40±0.06,亦明显低于模型组(P＜ 0.05、0.01).50 mg/kg和100 mg/kg青蒿琥酯组红斑评分、炎症细胞计数和MPO活性与阳性对照组差异均无统计学意义.结论 青蒿琥酯能抑制小鼠玫瑰痤疮样炎症反应,以中、高剂量最为明显.

Effects of artesunate on rosacea-like inflammation in mouse models

Objective To evaluate effects of artesunate on rosacea-like inflammation in mouse models.Methods Twenty-five male BALB/c mice aged 7 weeks were injected subcutaneously with 40 μ1 antibacterial peptide LL-37 into the back once every 12 hours for 4 sessions to establish mouse models with rosacea-like inflammation.These 25 mice were randomly and equally divided into 5 groups:after each injection of LL-37,model group were gavaged with sodium chloride physiological solution,treatment groups gavaged with 25,50 and 100 mg/kg artesunate solution separately,and positive control group gavaged with 30 mg/kg doxycycline hydrochloride solution.Another 5 healthy mice injected subcutaneously with pure water into the back for 4 sessions served as blank control group.Forty-eight hours after the initial injection of LL-37,changes in skin lesions and the intensity of erythema were assessed.Skin tissues at the dorsal injection site were resected and subjected to HE staining,the tissue structure was observed and the number of inflammatory cells was counted.Enzyme-linked immunosorbent assay (ELISA) was performed to estimate the activity of myeloperoxidase (MPO) in skin lesions.Results The model group showed obvious inflammatory reactions,and significantly increased erythema score (3.20 ± 0.84),inflammatory cell count (517.27 ± 99.43) and MPO activity (0.57 ± 0.08) compared with the blank control group (all P ＜ 0.01).The positive control group showed significantly decreased erythema score (1.60 ± 0.89),inflammatory cell count (270.93 ± 124.63) and MPO activity (0.40 ± 0.05) compared with the model group (P ＜ 0.05,0.01,0.01,respectively).Moreover,the erythema score,inflammatory cell counts and MPO activity were all significantly lower in 50-(1.80 ± 0.84,286.00 ± 33.72,0.43 ± 0.05,respectively) and 100-mg/kg artesunate groups (1.40 ± 0.55,258.00 ± 36.44,0.40 ± 0.06,respectively) than in the model group (P ＜ 0.05 or 0.01).However,there were no significant differences in the erythema score,inflammatory cell count and MPO activity between 50-or 100-mg/kg artesunate group and the positive control group (P ＞ 0.05).Conclusion Artesunate can inhibit rosacea-like inflammatory reactions in mouse models,especially the middle-and high-dose artesunate.