| nm23-H1 基因杂合性缺失及突变与大肠癌转移抑制 |
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| 引用本文： | 骆成玉,祝学光,李世拥,王申五. nm23-H1 基因杂合性缺失及突变与大肠癌转移抑制[J]. 中国普外基础与临床杂志, 1999, 6(3): 143-145 |
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| 作者姓名： | 骆成玉 祝学光 李世拥 王申五 |
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| 作者单位： | 1. 北京军区总医院普外科,北京,100700
2. 北京医科大学人民医院 |
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| 摘 要： | 应用Ｓｏｕｔｈｅｒｎ印迹杂交和ＲＴ－ＰＣＲ－ＳＳＣＰ银染，克隆及序列测定检测３６例大肠癌组织标本中ｎｍ２３－Ｈ１杂合性缺失和突变情况。结果：本组标本ｎｍ２３－Ｈ１杂合性缺失率为２９．６３％，Ｄｕｋｅ’ｓＤ期及远处转移大肠癌有较高的杂合性缺失发生率；大肠癌中未见ｎｍ２３－Ｈ１ＡＤ基因突变 。
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| 关 键 词： | 大肠癌 nm23－H1 基因突变 杂合性缺失 肿瘤转移 |
THE RELATION OF THE LOSS OF HETEROZYGOSITY AND MUTATION FOR nm23-H1 WITH THE INHIBITION OF METASTASIS IN COLORECTAL CARCINOMA |
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| Luo Chengyu,Zhu Xueguang,Li Shiyong,et al.. THE RELATION OF THE LOSS OF HETEROZYGOSITY AND MUTATION FOR nm23-H1 WITH THE INHIBITION OF METASTASIS IN COLORECTAL CARCINOMA[J]. Chinese Journal of Bases and Clinics In General Surgery, 1999, 6(3): 143-145 |
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| Authors: | Luo Chengyu Zhu Xueguang Li Shiyong et al. |
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| Affiliation: | Luo Chengyu,Zhu Xueguang,Li Shiyong,et al. Department of General Surgery,Beijing Army General Hospital,Beijing 100700 |
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| Abstract: | The loss of heterozygosity and mutation for nm23 H1 gene in colorectal carcinomas were studied by Southern blot and RT PCR SSCP/silver staining sequencing. The rate of loss of heterozygosity for nm23 H1 was 29.63%. The cases of Duke's stage D and distant metastatsis had higher frequency of the loss of heterozygosity. No mutation for nm23 H1 was found in colorectal carcinomas. These reaults indicate that the loss of heterozygosity for nm23 H1 may play a significant role in the malignant progression and distant metastasis in colorectal carcinomas. |
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| Keywords: | Colerectal neoplasm nm23 H1 gene Loss of heterozygosity Gene mutation |
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