Carbon Dioxide Water Bathing Enhances Myogenin but Not MyoD Protein
Expression after Skeletal Muscle Injury |
| |
| Authors: | Koji Nonaka Junichi Akiyama Naomi Tatsuta Satsuki Une Kenichi Ito Shinya Ogaya Masataka Kataoka Akira Iwata Kuniharu Okuda |
| |
| Affiliation: | 1) Department of Physical Therapy, Osaka Prefecture University;2) Research Institute of Health Welfare, Kibi International University;3) Department of Living Science, Shujitsu Junior College |
| |
| Abstract: | [Purpose] We reported that carbon dioxide (CO2) water bathing acceleratesskeletal muscle regeneration; however, the underlying mechanism was unclear. MyoD andmyogenin play roles in muscle regeneration, and the purpose of this study was to determinechanges in MyoD and myogenin caused by CO2 water bathing after injury.[Subjects] Sixteen female Wistar rats (n = 4 per group) were used. [Methods] The rats weredivided into four groups: no-injury (NI), injury (IC), injury + tap water bathing (ITW),and injury + CO2 water bathing (ICO2). Muscle injury was induced byinjection of bupivacaine hydrochloride into the left tibial anterior (TA) muscles. Tapwater and CO2 (1,000 ppm) water bathing were performed at 37 °C for 30 minutesonce a day. The left TA muscles were removed 4 days after injury, and the expressions ofMyoD and myogenin were measured. [Results] MyoD and myogenin were increased in the IC,ITW, and ICO2 groups compared with the NI group. Although the MyoD level wassimilar in the IC, ITW, and ICO2 groups, myogenin increased more in theICO2 group than in the IC and ITW groups. [Conclusion] CO2 waterbathing after muscle injury appears to induce an increase in the expression ofmyogenin. |
| |
| Keywords: | Skeletal muscle injury CO2 water bathing Myogenic regulatory factors |
|
|
