醒脑静乳剂解热镇痛抗炎作用及对中枢神经系统的影响

目的观察醒脑静乳剂镇痛、抗炎、解热、抗菌作用及对小鼠神经行为的影响，以为临床用药提供实验依据。方法采用小鼠扭体法、热板法观察醒脑静乳剂的镇痛作用；采用二甲苯诱导的小鼠耳肿胀模型观察醒脑静乳剂的抗炎作用；以细菌内毒素为致热源，观察醒脑静乳剂对家兔的解热作用；采用试管二倍稀释法测定醒脑静乳剂的最低抑菌浓度（MIC）以观察体外抗菌作用；通过检测小鼠自由活动次数，戊巴比妥钠诱导小鼠的睡眠时间以及士的宁诱发小鼠惊厥的发生率观察醒脑静乳剂对小鼠神经行为的影响。结果醒脑静乳剂可减少冰醋酸引起的小鼠扭体反应，并可延长小鼠热板舔足反应潜伏期，显著抑制二甲苯诱导的小鼠耳肿胀程度，降低细菌内毒素致热家兔体温的升高，且对球菌和杆菌均具有不同程度的体外抑菌作用。对中枢神经系统研究结果表明小剂量的醒脑静乳剂（205．5、102．75、51．4mg·kg^-1）能显著增加小鼠的自由活动数，并呈剂量依赖性，而高剂量的醒脑静乳剂（685mg·kg^-1）则使小鼠自由活动数明显减少（P〈0．01）；另外，小剂量的醒脑静乳剂（205．5、102．75、51．4mg·k^-1）能拮抗戊巴比妥钠诱导小鼠的睡眠时间（P〈0．01），而高剂量的醒脑静乳剂（685mg·kg^-1）则延长戊巴比妥钠诱导小鼠的睡眠时间（P〈0．01）。在士的宁致惊厥模型上发现小剂量的醒脑静乳剂（205．5、102．75、51．4mg·kg^-1）能明显提高小鼠惊厥的发生率，而高剂量的醒脑静乳剂（685mg·kg^-1）则对士的宁惊厥有拮抗作用，并能显著抑制惊厥的发生（P〈0．05）。结论醒脑静乳剂具有一定的镇痛、抗炎、解热、抗菌作用。另外，醒脑静乳剂对中枢神经起小剂量兴奋大剂量抑制的双向调节作用．并对柿惊厥也具有双向调节作用。

Study on the main pharmacological effects of xingnaojing emulsions

Aim This study was concerned with the effects of Xingnaojing emulsions （XNJE） on pain, inflammation, fever, bacteria multiplying and neural behavior of mice, for the instruction of reasonable clinical application. Method We took the method of writhing by glacial acetic acid and hot - plate in mice to study the analgesic effect of XNJE. To explore the anti-inflammatory actions of XNJE, acute inflammatory model of mice ear edema induced by dimethylbenzene was built. We used bacterial endotoxin as pyrogen to study the anti- pyretic effects of XNJE on rabbits. And we also adopted the method of double dilution to measure the minimum inhibitory concentration （MIC） of XNJE. TO observe effects of XNJE on neural behavior of mice,we measured the times of mice＇s spontaneous motion, Pentobar- bital Sodium-induced sleep time in mice and the incidence of Strychnine-induced convulsions in mice. Results We found that XNJE could reduce glacial acetic acid-induced writhing response in mice, extend the latency of licking paws response on hot plate in mice, significantly inhibit xylene-induced ear swelling in mice, reduce the rise of rabbit body temperature induced by bacterial endotoxin, and inhibit both cocci and bacilli＇s growth with different degrees in vitro. Study on the central nervous system showed that small doses of XNJE （205.5,102.75,51.4 mg · kg^-1） significantly increased the times of mice＇s spontaneous motion which took on a dose-dependent manner,while to the high-dose XNJN （685 mg·kg^-1）, mice＇s spontaneous motion was significantly reduced （P 〈0.01 ）;In addition, small doses of XNJE （205.5,102.75,51.4 mg· kg^- 1 ） could resist Pentobarbital Sodium-induced sleep time in mice （ P 〈 0.01 ）, and high- dose XNJE （685 mg·kg^-1 ） could extend pentobarbital sodium-induced sleep time in mice （P 〈 0.01 ）. At strychnine-induced convulsion model, small doses of XNJE （205.5,102.75,51.4 mg·kg^-1） could significantly increase the incidence of Strychnine-induced convulsions in mice,while high-dose XNJE （685 mg·kg^-1 ） could decrease the incidence of Strychnine-induced convulsions in mice and could significantly inhibit the occurrence of convulsion （ P 〈 0.05 ）. Conclusion XNJE has certain effects on analgesia, anti-inflammatory, antipyretic and anti-bacterial side. In addition, XNJE effects at a two-way regulation on the central nervous excitement that exciting with low-dose and inhibiting with high-dose. And XNJE＇s effect on anti-convulsion is also at a two-way regulation. As a conclusion, the results can provide useful information for clinical treatment.