| 线粒体DNA与人宫颈细胞癌变的关系 |
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| 引用本文： | 薛文群,陈道桢,杨幼易,耿金花,王峻峰,孟西娜.线粒体DNA与人宫颈细胞癌变的关系[J].中国优生与遗传杂志,2005,13(12):17-18. |
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| 作者姓名： | 薛文群 陈道桢 杨幼易 耿金花 王峻峰 孟西娜 |
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| 作者单位： | 南京医科大学附属无锡妇幼保健院,214002 |
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| 摘 要： | 目的探讨线粒体DNA与人宫颈细胞癌变的关系.方法采用PCR方法制备地高辛标记的3条人mtDNA探针,对一株人宫颈癌Hela细胞系、1例原代培养人皮肤成纤维细胞和24例子宫颈癌患者的活检癌组织及癌旁宫颈组织的染色体或间期细胞核进行了荧光原位杂交分析.结果 3条mtDNA探针在原代培养人皮肤成纤维细胞染色体或间期核中均未出现阳性杂交信号;而在人宫颈癌Hela细胞和24例肿瘤组织标本的部分染色体或间期细胞核中均出现了阳性杂交信号.而在癌旁组织的部分染色体或间期细胞核中虽也出现了阳性杂交信号.但与肿瘤组织标本相比有显著性差异.结论表明癌细胞核基因组中存在mtDNA的同源序列,这些同源片段的出现可能与细胞癌变有关.
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| 关 键 词： | 线粒体DNA探针 宫颈癌 核基因组 荧光原位杂交 |
| 文章编号： | 1006-9534（2005）12-0017-02 |
| 收稿时间： | 2005-07-07 |
| 修稿时间： | 2005年7月7日 |
Relationship between mitochondrion DNA(mtDNA) and carcinogenesis of human cervical cells |
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| XUE Wen-qun,CHEN Dao-zhen,YANG You-yi,GENG Jin-hua,WANG Jun-feng,MENG Xi-na.Relationship between mitochondrion DNA(mtDNA) and carcinogenesis of human cervical cells[J].Chinese Journal of Birth Health & Heredity,2005,13(12):17-18. |
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| Authors: | XUE Wen-qun CHEN Dao-zhen YANG You-yi GENG Jin-hua WANG Jun-feng MENG Xi-na |
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| Institution: | Wuxi Hospital for Matemaland Child Health Care Affiliated Medical School of Nanjin , Wuxi Jiangsu 214002 P . R . China |
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| Abstract: | Objective: To explore the relationship between mitochondrion DNA(mtDNA) and carcinogenesis of human Cervical cells.Methods:Three kinds of non-radioactive DIG-labelled.mtDNA probes were prepared by polymerase chain reaction(PCR).The chromosomes in metaphase or interphase nuclei of one fibrobasts and Hela cell line and 24 cervical carcinomas together with the adjacent normal tissues were analyzed by fluorescence in situ hybridization(FISH).Results:Hybridization signals were4 partly observed on the chromosomes in metaphase or interphase nuclei of Hela cell line and 24 cervical carcinomas together with the adjacent normal tissues but not in fibrobasts.Conclusion:The mtDNA homology sequence exists in the nuclear genome of carcinomas cells and it might exert important effects on the cqarcinogenic process. |
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| Keywords: | Mitochondrion DNA probe Cervical carcinomas Nnuclear genome Fluorescence in situ hybridization |
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