五子降糖方对T2DM模型大鼠骨骼肌GLUT4蛋白表达的影响

目的: 观察五子降糖方对2型糖尿病模型大鼠血糖及骨骼肌葡萄糖转运蛋白4(GLUT4)表达的影响,初步探讨其改善糖代谢的分子机制。 方法: 采用高脂饮食喂养6周联合ip小剂量链脲佐菌素(STZ)的方法建立2型糖尿病大鼠模型,随机分为模型组、二甲双胍(0.1 g·kg^-1)组、五子降糖方低、中、高剂量组(3.5,7.0,14.0 g·kg^-1),另设正常组,各组大鼠分别ig相应的药物,容量为7.5 mL·kg^-1。模型组及正常组ig纯净水;二甲双胍组ig盐酸二甲双胍混悬液;五子降糖方低、中、高剂量组ig五子降糖方混悬液,干预6周。分别在ig前、ig后2周、4周、6周测空腹血糖及餐后2 h血糖。ig后6周处死大鼠,取其左后肢大腿股直肌组织,检测GLUT4蛋白的表达。 结果: 与正常组比较,模型组T2DM大鼠空腹和餐后2 h血糖升高,骨骼肌GLUT4蛋白表达水平降低,差异均具有统计学意义(P<0.05);五子降糖方能够降低T2DM大鼠空腹血糖及餐后2 h血糖水平,提高T2DM大鼠骨骼肌GLUT4蛋白表达水平,差异均具有统计学意义(P<0.05)。 结论: 五子降糖方可降低T2DM模型大鼠空腹及餐后2 h血糖,作用机制可能与提高骨骼肌GLUT4蛋白表达水平有关。

Effects of Wuzi Jiangtang Fang on the GLUT4 Expression in Skeleton Muscle of Type 2 Diabetic Rats

Objective: To observe the effects of Wuzi Jiangtang Fang on blood glucose and the glucose transporter 4(GLUT4) expression in skeleton muscle of type 2 diabetic model rats to explore the molecular mechanism of improving glucose metabolism. Method: The rat model of type 2 diabetes was established by feeding with high-fat diet for 6 weeks and intraperitoneal injection of small dose of streptozotocin(STZ). The diabetic rats were randomly divided into model group administrated with purified water, metformin group with metformin suspension, Wuzi Jiangtang Fang groups with water extracts of different doses respectively. Meanwhile, normal group was treated with purified water. The dose was 7.5 mL·kg^-1 of body weight every day. The interventions were administrated by ig for 6 weeks. The fasting blood glucose and 2 h postprandial blood glucose were detected at the beginning of the treatment, and at the ends of the second, the fourth and the sixth week during the 6 weeks' treatment period respectively. At the end of the sixth week, the skeleton muscle was taken for the measurement of GLUT4 protein expression. Result: Compared with the normal group, the blood glucose levels of type 2 diabetes mellitus(T2DM) rats were elevated and the GLUT4 expression of skeleton muscle was decreased in T2DM model rats, with significant differences in all of the items above (P<0.05); Wuzi Jiangtang Fang reduced the fasting blood glucose and 2 h postprandial blood glucose levels of T2DM model rats, and improved the GLUT4 expression in skeleton muscle of T2DM model rats. The differences regarding the item examined above all had statistical significance (P<0.05). Conclusion: Wuzi Jiangtang Fang can decrease the fasting blood glucose and 2 h postprandial blood glucose of T2DM model rats, which may be related with the improvement of GLUT4 expression in skeleton muscle of T2DM model rats.