地黄饮子对卒中后抑郁大鼠海马5-羟色胺受体的影响

目的:通过观察地黄饮子对卒中后抑郁大鼠5-羟色胺1A受体(5-HT1AR)与5-羟色胺2A受体(5-HT2AR)mRNA水平表达的影响,进一步探讨地黄饮子治疗脑卒中后抑郁的可能作用机制.方法:健康成年SD大鼠60只,雌雄各半,随机分为6组(空白组、模型组、百优解组、地黄饮子高、中、低剂量组)每组10只,除空白对照组每笼饲养5只外(雌雄分开),其余各组均采用单独饲养法,每笼饲养1只.卒中后抑郁模型建立成功后,模型组、氟西汀组和地黄饮子高、中、低剂量组分别以2.0mL蒸馏水、盐酸氟西汀(1.8 mg·kg-1)和地黄饮子(按生药)450,150,75 mg· kg-ig,1次/d,持续用药至行为学检测.在脑卒中后抑郁大鼠模型上,采用实时荧光定量RT-PCR方法,对卒中后抑郁大鼠5-HT1AR与5-HT2AR mRNA水平进行观察.结果:大鼠海马5-HT1AR相对表达水平与空白组(1.12±0.16)比较,模型组(0.23 ±0.13)有显著性差异(P＜0.01);地黄饮子中、高剂量组(0.76 ±0.13,0.75 ±0.11)与模型组比较,有显著性差异(P＜0.05).各组大鼠海马5-HT2AR mRNA表达水平与空白组(1.13 ±0.12)比较,模型组(2.21±0.21)有显著性差异(P＜0.05);地黄饮子高、中剂量组(1.21±0.21,1.25 ±0.26),与模型组比较,有显著性差异(P＜0.05).结论:地黄饮子可能是通过上调5-HT1AR mRNA、下调5-HT2AR mRNA在海马区的表达,达到治疗卒中后抑郁的目的.

Influence of Dihuang Yinzi on the Content of Serotonin Receptors in Hippocampal of Rat with Poststroke Depression

Objective: To observe influence of of Dihuang Yizi (DHYZ) on serotonin 1 A receptor (5-HT_1AR) and serotonin 2 A receptor (5-HT_2AR) mRNA express in poststroke depression rats, and further discuss mechanism. Method: The sixty healthy adult SD rats were randomly divided into 6 groups including blank control group, model group, the optimal solutions group, DHYZ high dose group, medium group, low group. Afterpoststroke depression model was established the corresponding drugs were given (hydrochloric acid prozac,DHYZ 450,150,75 mg·kg^-1. The real-time fluorescent quantitative RT-PCR method was used to assay 5-HT_1AR and 5-HT_2AR mRNA expression. Result: Compared with model group; DHYZ medium (0.76± 0.13), (1.21±0.21) and high dose groups (0.75±0.1), (11.25±0.26) increased the 5-HT_1AR mRNA expression, and decreased the 5-HT_2AR mRNA expression (P<0.05). Conclusion: DHYZ may raise 5-HT_1AR mRNA, and decrease 5-HT_2AR mRNA, which can explain mechanism of DHYZ in treatment of poststroke depression.