高效液相色谱校正因子法测定苦碟子提取物中5种主要成分的含量

目的通过测定苦碟子提取物中咖啡酸、菊苣酸、木犀草素-7-O-β-D-葡萄糖苷-(1→6)-β-D-葡萄糖苷、芹菜素-7-O-β-D-葡萄糖醛酸苷与木犀草素-7-O-β-D-葡萄糖醛酸苷的校正因子,建立以1种对照品测定苦碟子提取物中5种主要成分的含量的方法。方法以Agilent TC-C18(250 mm×4.6 mm,5μm)为分析柱,柱温30℃,流动相为甲醇(A)-0.05%磷酸(B),梯度洗脱:0~10 min:30%A,10~30 min:30%~50%A,流速1.2 mL.min-1,检测波长350 nm。结果咖啡酸、菊苣酸、木犀草素-7-O-β-D-葡萄糖苷-(1→6)-β-D-葡萄糖苷、芹菜素-7-O-β-D-葡萄糖醛酸苷的校正因子分别为:1.129、0.873 3、1.320、1.129(RSD=1.6%~2.1%),苦碟子提取物中咖啡酸、菊苣酸、木犀草素-7-O-β-D-葡萄糖苷-(1→6)-β-D-葡萄糖苷、木犀草素-7-O-β-D-葡萄糖醛酸苷与芹菜素-7-O-β-D-葡萄糖醛酸苷的含量分别为4.2%、29.5%、6.5%、23.0%、5.0%。结论本法简便,灵敏,重现性好,可用于苦碟子提取物和制剂的定量分析及质量控制。

Relative correction factor determination of 5 major compounds in the extract from Ixeris Sonchifolia Hance by HPLC

Objective To determine the relative correction factors（RCF） between luteolin-7-O-β-D-glucuronopyranoside and another 4 phenolic acids：caffeic acid,cichoric acid,luteolin-7-O-β-D-glucopyranoside（1→6）-β-D-glucopyranoside,and apigenin-7-O-β-D-glucuronopyranoside.All the 5 compounds in the extract from Ixeris Sonchifolia Hance were determined by only one reference substance.Methods The Agilent TC-C18 column（250 mm×4.6 mm,5 μm）was 30 ℃,the mobile phase was composed of methanol（A） and 0.05% phosphoric acid（B）：0-10 min,30%A,10-30 min,30%-50%A.The flow rate was 1.2 mL·min-1,and the detection wavelength was 350 nm.Results The relative correction factors of caffeic acid,cichoric acid,luteolin-7-O-β-D-glucopyranoside（1→6）-β-D-glucopyranoside,and apigenin-7-O-β-D-glucuronopyranoside were identified as 1.129,0.873 3,1.320,and 1.129（RSD=1.6%-2.1%）.The content of the 5 compounds were 4.2%,29.5%,6.5%,23.0%,and 5.0%.Conclusion The method is simple,accurate,sensitive and reproducible,which can be used in the quantitative analysis and quality control of the extract and preparation of Ixeris Sonchifolia Hance.