|
|||||
|
|
| 硬质合金粉尘生物学作用的体外研究 | |
| 引用本文： | 邹彤彤,邹世渠,吴建明,沙斌,韩秀荣. 硬质合金粉尘生物学作用的体外研究[J]. 中国职业医学, 1993, 0(2) |
| 作者姓名： | 邹彤彤 邹世渠 吴建明 沙斌 韩秀荣 |
| 作者单位： | 北京医科大学公共卫生学院，北京医科大学公共卫生学院，北京医科大学公共卫生学院，北京医科大学公共卫生学院，北京医科大学公共卫生学院 北郊学院路 100083，北郊学院路 100083，北郊学院路 100083，北郊学院路 100083，北郊学院路 100083 |
| 摘 要： | 本文应用体外细胞培养技术,通过测定豚鼠肺泡巨噬细胞培养液中乳酸脱氢酶活性、人胚肺二倍体成纤维细胞的DNA合成及扫描电镜观察,研究了我国有代表性的4种硬质合金粉尘的细胞毒性。结果表明,硬质合金粉尘可引起乳酸脱氢酶活性一定程度升高,和成纤维细胞DNA合成不同程度降低,以及细胞表面形态学改变,说明硬质合金粉尘具有一定的生物学作用,其细胞毒性明显低于二氧化硅粉尘。 |
| 关 键 词： | 硬质合金粉尘 肺泡巨噬细胞 细胞毒性 |
The biological effect of hard metal dusts in vitro |
|
| Zou Tongtong,et al.. The biological effect of hard metal dusts in vitro[J]. China Occupational Medicine, 1993, 0(2) | |
| Authors: | Zou Tongtong et al. |
| Abstract: | The cytotoxicities of four representative hard metal dusts were studied in vitro by determining the activities of lactic dehydrogenase (LDH) in the culture medium of the alveolar macrophages of guinea pigs and ~5H-thymidine incorporation to DNA of fibroblasts. The morphology changes of the macrophages were also observed by the scanning electron microscopy. The results indicated an increase of LDH activity in case of exposing to hard metal dusts, except tungsten carbide (WC) and a decrease of DNA synthesis in two kinds of hard metal dusts in certain extent. It suggested that hard metal dusts might elicit some cytotoxicity but significantly lower than that of SiO_2. |
| Keywords: | hard metal dust alveolar macrophage cytotoxicity |
| 本文献已被 CNKI 等数据库收录！ | |