血管性痴呆小鼠海马NOS活力和nNOS蛋白表达的改变

目的:观察血管性痴呆(VD)小鼠海马内一氧化氮合酶(NOS)的活性和神经元型一氧化氮合酶(nNOS)阳性神经元的表达,探讨VD的发生机制. 方法:复制小鼠VD模型,利用Y-迷宫检测VD模型小鼠学习记忆能力,采用NADPH-d组织化学和nNOS免疫组织化学方法,研究VD小鼠与正常小鼠海马NOS和nNOS阳性神经元数量的变化. 结果:VD小鼠比正常小鼠Y-迷宫学习记忆训练次数明显增多,差异有显著性(P<0.01);海马CA1区NOS和 nNOS阳性神经元的数量明显增多,差异有统计学意义(P<0.05). 结论:VD的发生可能与海马NOS和 nNOS阳性神经元的数量增多有关.

Changes of NOS activity and Nnos expression in hippocampus of vascular dementia mice

AIM: To observe the activity changes of nitric oxide synthase(NOS) and the expression level of neuronal nitric oxide synthase(nNOS) in the hippocampus of vascular dementia(VD) mice and to investigate the mechanism underlying VD.METHODS: Models of VD mice were made.NADPH-d histochemistry and nNOS immunohistochemistry were used to investigate the changes of NOS in different groups and Y-maze test was conducted to observe the changes of their learning and memory abilities.RESULTS: Compared with those of the control group,the learning and memory abilities of the VD group significantly decreased(P<0.01) and the number of NOS positive neurons and nNOS positive neurons in the hippocampus significantly increased(P<0.05).CONCLUSION: VD is probably related to the increase of the numbers of NOS positive neurons and nNOS positive neurons in the hippocampus.