200404245

目的：体外研究内皮素-1对黑素细胞黏附和迁移的作用。方法：用内皮素-1(ET-1)处理人表皮纯化培养的黑素细胞，观察其在纤维连接素包裹的培养板上的黏附以及通过微孔滤膜的情况，并与干细胞因子及未加因子组作对照比较。结果：与干细胞因子组相比，内皮素-1处理的黑素细胞更易黏附于培养板和通过微孔滤膜，尤其是当其浓度在32 nmol/L时，而它的浓度超过128 nmol/L时，对黑素细胞的黏附和迁移却呈现抑制作用。除了2 nmol/L组，其他任何浓度下内皮素-1均对黑素细胞黏附起促进作用(P<0.01)。但对黑素细胞迁移来说，每个观察浓度下的内皮素-1均表现为迁移促进作用，且这种作用强于干细胞因子处理的黑素细胞 (P<0.01)。结论：内皮素-1可以影响黑素细胞的黏附和迁移，这可以部分解释内皮素-1对白癜风皮损区黑素细胞的功能有调节作用。

Effects of endothelin-1 on melanocyte adhesion and migration

Objective To investigate the effect of endothelin-1 (ET-1) on the adhesion and migration of melanocyte in vitro.Methods Human epidermal melanocytes that had been cultured and purified were treated with ET-1 and observed for adhesion to bovine serum fibronectin-coated culture dishes. Stem cell factor (SCF) and ET-1 treated cells were also examined for migration into micropore filters coated with the same protein. Results Compared with the SCF group, ET-1 treated melanocytes were easier to adhere to the dishes and to be moved into the filters, especially when the concentration was 32 nmol/L. When the concentration of ET-1 was 128 nmol/L or more, it could inhibit the adhesion and migration of melanocyte.At any concentration of ET-1 except at 2 nmol/L, there was a significant difference between ET-1-treated and untreated melanocytes (P<0.01) when the adhesion test was carried out.However, even at 2 nmol/L ET-1, there was obviously increased migration compared with those of ET-1 untreated melanocytes and SCF-treated cells (P<0.01).Conclusion ET-1 may influence the adhesion and migration of melanocyte, which can partly explain the capacity of ET-1 to regulate the melanocyte function in vitiligo lesions.