| 联合应用Ag85A和GM-CSF DNA疫苗对小鼠移植性膀胱癌BTT739细胞瘤免疫治疗的研究 |
| |
| 引用本文： | 张辉,宋永胜,商超. 联合应用Ag85A和GM-CSF DNA疫苗对小鼠移植性膀胱癌BTT739细胞瘤免疫治疗的研究[J]. 现代肿瘤医学, 2011, 19(6): 1049-1051. DOI: 10.3969/j.issn.1672-4992.2011.06.02 |
| |
| 作者姓名： | 张辉 宋永胜 商超 |
| |
| 作者单位： | 1. 中国医科大学附属盛京医院泌尿外科,辽宁,沈阳,110004
2. 中国医科大学基础医学院神经生物学教研室,辽宁,沈阳,110001 |
| |
| 基金项目： | 国家自然科学基金资助项目，辽宁省科学技术基金资助项目 |
| |
| 摘 要： | 目的:探讨联合应用Ag85A与GM-CSF DNA疫苗对小鼠移植性膀胱癌BTT739细胞瘤免疫治疗的效果。方法:选用小鼠膀胱癌BTT739细胞株,构建T739小鼠移植瘤模型。随机数字表法分为5组:生理盐水组、空载体组、Ag85A组、联合应用组和BCG组。荷瘤后第7天、14天和21天各肌注1次,末次注射后第7天检测各组小鼠脾脏的CD4+和CD8+T细胞亚群数量及CD4+/CD8+比值、血清IFN-γ和肿瘤重量。结果:与生理盐水组对比,单独应用Ag85A和联合应用Ag85A与GM-CSF DNA疫苗均能够升高CD4+和CD8+T细胞的百分比及CD4+/CD8+的比值,提高血清IFN-γ浓度,降低移植瘤重量,差异具有统计学意义(P<0.05)。联合应用的效果要明显优于单独应用组,差异具有统计学意义(P<0.05),但尚不及单纯应用BCG的免疫治疗效果。结论:联合应用Ag85A与GM-CSF DNA疫苗能够提高荷瘤小鼠的免疫功能,从而达到了抑制肿瘤生长的作用,其效果明显优于单独应用Ag85A DNA疫苗,二者联用具有协同增强免疫效应。
|
| 关 键 词： | 膀胱癌 DNA疫苗 卡介苗 Ag85A GM-CSF |
The immunological therapy effect of combined Ag85A and GM-CSF DNA vaccine in bladder tumor-bearing mice cells |
| |
| ZHANG Hui,SONG Yong-sheng,SHANG Chao. The immunological therapy effect of combined Ag85A and GM-CSF DNA vaccine in bladder tumor-bearing mice cells[J]. Journal of Modern Oncology, 2011, 19(6): 1049-1051. DOI: 10.3969/j.issn.1672-4992.2011.06.02 |
| |
| Authors: | ZHANG Hui SONG Yong-sheng SHANG Chao |
| |
| Affiliation: | ZHANG Hui1,SONG Yong-sheng1,SHANG Chao21Department of Urinary Surgery,the Affiliated Shengjing Hospital,Chian Medical University,Shenyang 110004,China,2Department of Neurobiology,College of Basic Medical Science,China Medical University,Shenyang 110001,China. |
| |
| Abstract: | Objective:To explore the immunological therapeutic effect of combined Ag85A and GM-CSF DNA vaccine in bladder tumor-bearing mice cells.Methords: T739 mice with bladder cancer were constructed by injecting mice bladder cancer cell strain BTT739 subcutaneously.The mice were divided randomly into five groups :NS,pcDNA3.1,Ag85A,Ag85A+GM-CSF and BCG.DNA vaccines were injected subcutaneously the 7th day,the 14th day,and the 21th day respectively.After 7 days,the number of CD4+ and CD8+ T cell subsets was detected... |
| |
| Keywords: | Ag85A GM-CSF |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
