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| 尼美舒利对不同COX-2表达水平的食管鳞癌细胞的抑制作用 | |
| 引用本文： | 孟欣颖,赵燕,朱圣韬,张澍田. 尼美舒利对不同COX-2表达水平的食管鳞癌细胞的抑制作用[J]. 现代肿瘤医学, 2011, 19(5): 848-851. DOI: 10.3969/j.issn.1672-4992.2011.05.006 |
| 作者姓名： | 孟欣颖 赵燕 朱圣韬 张澍田 |
| 作者单位： | 1. 青岛市市立医院(东区)消化内科,山东,青岛,266071 2. 首都医科大学附属北京友谊医院消化内科,北京,100050 |
| 摘 要： | 目的:比较选择性环氧合酶-2(COX-2)抑制剂尼美舒利对不同COX-2表达水平的食管鳞癌细胞的抑制作用。方法:选取食管鳞癌细胞株EC 109、KYSE 150和TE-1,采用Western blot方法测定COX-2蛋白表达、MTT法检测细胞增殖抑制,流式细胞术检测细胞周期和细胞凋亡,观察尼美舒利对各组细胞的增殖抑制和促凋亡作用。结果:COX-2蛋白在EC 109细胞中呈高表达,KYSE 150细胞中呈中等度表达,TE-1细胞不表达COX-2蛋白。尼美舒利在50μmol/L-400μmol/L浓度区间可抑制EC 109、KYSE 150细胞的增殖(P<0.05),并呈剂量依赖性,在400μmol/L时对TE-1细胞有抑制作用。EC 109细胞尼美舒利的IC50值最低,KYSE150次之,TE-1最高。尼美舒利可使EC 109和KYSE 150的细胞周期阻滞于G0/G1期,并诱导细胞凋亡,但对TE-1细胞无上述作用。结论:尼美舒利对表达COX-2的食管鳞癌细胞有较好的增殖抑制和促凋亡作用。 |
| 关 键 词： | 环氧合酶-2 食管鳞癌 增殖 凋亡 |
Inhibitory effect of Nimesulide on esophageal squamous cell carcinoma cell lines with different level of Cyclooxygenase-2 expression |
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| MENG Xin-ying,ZHAO Yan,ZHU Sheng-tao,ZHANG Shu-tian. Inhibitory effect of Nimesulide on esophageal squamous cell carcinoma cell lines with different level of Cyclooxygenase-2 expression[J]. Journal of Modern Oncology, 2011, 19(5): 848-851. DOI: 10.3969/j.issn.1672-4992.2011.05.006 | |
| Authors: | MENG Xin-ying ZHAO Yan ZHU Sheng-tao ZHANG Shu-tian |
| Affiliation: | MENG Xin-ying1,ZHAO Yan1,ZHU Sheng-tao2,ZHANG Shu-tian2 1Department of Digestive Diseases,Qingdao Municipal Hospital(East),Qingdao 266071,China,2Department of Digestive Diseases,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China. |
| Abstract: | Objective:To investigate the inhibitory effect of Nimesulide,a selective Cyclooxygenase-2(COX-2) inhibitor,on esophageal squamous cell carcinoma(ESCC) cell lines with or without COX-2 expression.Methods:The human ESCC cell lines EC 109,KYSE 150 and TE-1 were selected in this study.COX-2 expression was detected by Western blot.After 72 hours treatment with Nimesulide,the cell growth inhibition was determined by MTT assay,the cell cycle arrest and apoptosis were detected by flow cytometry.Results:COX-2 protei... |
| Keywords: | Cyclooxygenase-2 esophageal squamous cell carcinoma proliferation apoptosis |
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