| Changes of proton transportation across the inner mitochondrial membrane and H~+-ATPase in endotoxic shock rats |
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| 作者姓名： | 陆松敏 宋双明 刘建仓 杨鹤鸣 李萍 王正国 |
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| 作者单位： | Research Institute of Surgery，Third Military Medical University，Research Institute of Surgery，Third Military Medical University，Research Institute of Surgery，Third Military Medical University，Surgery Department of 306 Hospital，Research Institute of Surgery，Third Military Medical University，Research Institute of Surgery，Third Military Medical University Chongqing 400042，China，Chongqing 400042，China，Chongqing 400042，China，Bejing 100101，China，Chongqing 400042，China，Chongqing 400042，China |
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| 摘 要： | Protonelectrochemicalgradient (ΔμH+)intheinnermitochondrialmembrane (IMM )isthepowertosynthesizeATP .TransmembraneprotontranslocationisthebasistoformΔμH+.Thereisapaucityofliteratureconcerningthetransmembraneprotontranslocationinendotoxicshock .ThisstudyistoelucidatethechangesandmechanismofthetransmembraneprotontranslocationandH+ ATPaseactivityofthehepatocytemitochondriainendotoxicshock .METHODSEightyhealthyadultWistarratsweighing 2 5 0g± 5 0g ,eithersex ,weredividedrandomlyintobas…
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Changes of proton transportation across the inner mitochondrial membrane and H~+-ATPase in endotoxic shock rats |
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| LU Song min ,SONG Shuang ming,LIU Jian cang,YANG He ming,LI Ping and WANG Zheng guo Research Institute of Surgery,Third Military Medical University,Chongqing ,China.Changes of proton transportation across the inner mitochondrial membrane and H~+-ATPase in endotoxic shock rats[J].Chinese Journal of Traumatology(English Edition),2003(5). |
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| Authors: | LU Song min SONG Shuang ming LIU Jian cang YANG He ming LI Ping and WANG Zheng guo Research Institute of Surgery Third Military Medical University Chongqing China |
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| Institution: | LU Song min *,SONG Shuang ming,LIU Jian cang,YANG He ming,LI Ping and WANG Zheng guo Research Institute of Surgery,Third Military Medical University,Chongqing 400042,China |
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| Abstract: | Objective: To investigate the changes of proton transportation across the inner mitochondrial membrane (IMM) and H + ATPase of hepatocytes in endotoxic shock rats. Methods: Endotoxin from E.Coil of 5.0 mg/kg or saline of 1 ml/kg was injected into the femoral vein. The rats were sacrificed pre injection and 1, 3, 5, 8 hours after injection, and plasma and liver tissue samples were collected respectively. The liver tissue samples were used for preparation of mitochondria and submitochondrial particles (SMPs). The proton translocation of SMPs and H + ATPase, phospholipase A 2 (PLA 2) activities and malondialdehyde (MDA) content, membrane fluidities of different level of mitochondria membrane and plasma MDA content were assayed. Results: (1) Five hours after E. Coli. O111B4 injection, the maximum fluorescence quenching ACMA after adding ATP, nicotinamide adenin dinucleoacid hydrogen (NADH), and the succinate were significantly decreased (P< 0.05 ). The time of maximum fluorescent quenching and the half time of fluorescent quenching were significantly prolonged (P< 0.01 ), especially when NADH was used as a substrate. (2) The mitochondrial H + ATPase activity was significantly increased at early stage of endotoxic shock (P< 0.05 ), and significantly decreased at late stage of endotoxic shock (P< 0.01 ). (3) The mitochondrial membrane bound PLA 2 activity, plasmal and mitochondrial MDA content were significantly increased and succinate dehydrogenase (SDH) activity of mitochondria decreased markedly in endotoxic shock rats (P< 0.05 ). (4) The mitochondrial membrane fluidity of different lipid regions was decreased, especially in the head of phospholipid. Conclusions: Proton transportation across IMM and mitochondrial H + ATPase activity are significantly decreased in endotoxic shock. |
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| Keywords: | Mitochondria Shock septic H + ATPase |
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