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| 克雷伯氏肺炎杆菌生产1,3-丙二醇代谢途径中的酶活变化 | |
| 引用本文： | 王娅君,赵莉,徐佳杰,付水林,宫衡. 克雷伯氏肺炎杆菌生产1,3-丙二醇代谢途径中的酶活变化[J]. 医学教育探索, 2007, 0(4): 481-484 |
| 作者姓名： | 王娅君 赵莉 徐佳杰 付水林 宫衡 |
| 作者单位： | 华东理工大学生物反应器工程国家重点实验室; |
| 摘 要： | 考察了不同条件下克雷伯氏肺炎杆菌(Klebsiella.pneumoniae)合成1,3-丙二醇(1,3-PD)的3种关键酶(甘油脱氢酶(GDH)、1,3-PD氧化还原酶(PDOR)及甘油脱水酶(GDHz))的表观酶活变化。结果显示:这3种酶的酶活变化与Klebsiella.pneumoniae的1,3-PD代谢不完全相关。用SDS-PAGE电泳分析上述不同发酵条件下酶活变化,结果显示不同于3种酶的蛋白条带,Mr约为4.0×104位置的蛋白条带有明显的变化,这可能与菌体代谢过程相关,结论有待进一步深入研究。 |
| 关 键 词： | 克雷伯氏肺炎杆菌 甘油脱氢酶 1 3-丙二醇氧化还原酶 甘油脱水酶 |
Changes in the Activities of Metabolic Enzymes in Klebsiella pneumoniae during 1,3-Propanediol Production |
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| WANG Ya-jun,ZHAO Li,XU Jia-jie,FU Shui-lin,GONG Heng. Changes in the Activities of Metabolic Enzymes in Klebsiella pneumoniae during 1,3-Propanediol Production[J]. Researches in Medical Education, 2007, 0(4): 481-484 | |
| Authors: | WANG Ya-jun ZHAO Li XU Jia-jie FU Shui-lin GONG Heng |
| Affiliation: | State Key Laboratory of Bioreactor Engineering;East China University of Science and Technology;Shanghai 200237;China |
| Abstract: | The activities of the three enzymes(glycerol dehydrogenase(GDH),glycerol dehydratase(GDHt),1,3-propanediol oxidoreductase(PDOR)),considered as key metabolic enzymes for 1,3-propanediol formation in Klebsiella.pneumoniae were investigated at different experimental conditions in this work.Results showed that the variety of three enzymes were not completely interrelated with the 1,3-PD production in Klebsiella.pneumoniae.The expression patterns of enzymes under different culture conditions were analyzed by usi... |
| Keywords: | Klebsiellalebsiella.pneumoniae glycerol dehydrogenase 1 3-propanediol oxidoreductase glycerol dehydratase |
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