纳米锶磷灰石的体外细胞毒性研究

目的对纳米锶磷灰石进行体外细胞毒性试验,评价其生物安全性,为将其应用于骨折临床修复奠定基础。方法将不同浓度的纳米锶磷灰石浸提液与小鼠成纤维细胞L929体外共培养2d、4d、7d,倒置相差显微镜下观察细胞的形态变化;用四氮唑盐比色法(MTT)检测,计算细胞相对增殖度,用六级毒性分类法进行评级。结果培养期细胞贴壁生长,形态良好,随着培养天数的增加细胞大量增殖,毒级为0～1级。相同时间点不同浓度的纳米锶磷灰石浸提液的吸光度值无明显差异性(p>0.05)。结论初步证实纳米锶磷灰石具有人体应用的生物安全基础,无细胞毒性。

In Vitro Study of Cytotoxicity of Nano-fermorite

Objective Nano- fermorite was tested by in vitro cytotoxicity as first step for future use of this material as orthopedic implant. Methods Mouse fibroblast L929 cells were cultured in the medium of extraction of nano- fermorite at 100 %, 50 % and 0 % (control) respectively for 2, 4, 7days. The morphologic change of the cells were observed by the inverted phase contrast microscope. Cell growth was tested by MTT assay and the cell relative growth rate(RGR) was calculated. The cytotoxicity was graded by generally accepted standard. Results During all culture periods, L929 cells showed normal morphology and proliferation increased with culture time. The toxicity gradation of all groups was 0-1. The OD value of cells was similar for the negative control and the extracts (p>0.05). Conclusion The results indicate that nano- fermorite is compatible with the tested cells.