Gli1和PDGF-D在结直肠癌中的表达及其临床意义

  目的  探讨Gli1和PDGF-D在结直肠癌中的表达及其临床意义。  方法  采用免疫组织化学S-P法、Western blot法分别定性、定量检测54例结直肠癌组织及其对应30例癌旁正常组织中Gli1和PDGF-D蛋白表达情况并结合临床病例资料进行分析。  结果  免疫组织化学结果显示Gli1和PDGF-D在结直肠癌中的阳性表达率分别为(72.22%、74.07%), 与正常组织的阳性表达率(6.67%、33.33%)相比显著增高, 差异具有统计学意义(P < 0.05)。Western blot检测Gli1和PDGF-D在癌组织中相对表达量分别为0.731±0.238和0.817±0.288, 与正常组织0.196±0.041和0.236±0.060相比, 两者表达也显著增高, 差异具有统计学意义(P < 0.05);Gli1和PDGF-D的表达与肿瘤的分化程度、淋巴结转移有关(P < 0.05), PDGF-D还与肿瘤分期有关(P < 0.05);两者表达与患者的年龄、性别、肿瘤大小、侵袭深度均无明显相关性(P > 0.05);Gli1和PDGF-D在结直肠癌中的表达呈正相关(P < 0.05)。  结论  Gli1和PDGF-D在结直肠癌中异常高表达, 可能共同参与并促进结直肠癌的恶性进程。 

Expression of Gli1 and PDGF-D in colorectal cancer and its clinical significance

  Objective  This work aimed to investigate the expression of the Gli1 and PDGF-D proteins in colorectal cancer and its clinical significance.  Methods  Immunohistochemistry and Western blot analysis were used to detect the expression of Gli1 and PDGF-D in tissue samples from 54 colorectal cancer case patients and adjacent normal tissues from 30.The Results were obtained after clinicopathological features were analyzed.  Results  Immunohistochemical assay showed that the positive rates of Gli1 and PDGF-D in the colorectal cancer tissue samples(72.22% and 74.07%, respectively) were significantly higher than those in the normal tissues(6.67% and 33.33%, respectively)(P < 0.05).Western blot analysis revealed that the relative expression levels of Gli1 and PDGF-D in the colorectal cancer tissue samples(0.731±0.238 and 0.817±0.288, respectively) were also significantly higher than those in the normal tissue samples(0.196 ± 0.041 and 0.236±0.060, respectively)(P < 0.05).The expression of Gli1 and PDGF-D was positively correlated with the differentiation of colorectal cancer and lymph node metastasis, and the expression of PDGF-D was also positively correlated with the World Health Organization tumor-node-metastasis staging system(P < 0.05).Neither gene was correlated with patient sex, patient age, tumor size, and depth of tumor invasion(P > 0.05).The expression of Gli1 and that of PDGF-D were positively correlated(P < 0.05).  Conclusion  Gli1 and PDGF-D are expressed in colorectal cancer at abnormally high levels.They may act in concert and play an important role in the carcinogenesis of the disease.